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| Autor(es): Mostrar menos - |
Petean, Igor Bassi Ferreira
;
Silva-Sousa, Alice Correa
;
Maranon-Vasquez, Guido Artemio
;
de Paula-Silva, Francisco Wanderley Garcia
;
Kuechler, Erika Calvano
;
Antunes, Leonardo Santos
;
Segato, Raquel Assed Bezerra
;
da Silva, Lea Assed Bezerra
;
Mazzi-Chaves, Jardel Francisco
;
Lopes-Olhe, Fabiane Carneiro
;
Sousa-Neto, Manoel Damiao
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ARCHIVES OF ORAL BIOLOGY; v. 169, p. 8-pg., 2024-10-19. |
| Resumo | |
Objectives: To investigate the association between genetic polymorphisms in suppressor of cytokine signaling-1 ( SOCS-1 ) , tumor necrosis factor-alpha ( TNF-alpha ) and its receptors 1 and 2 ( TNFRSF1A and TNFRSF1B), receptor activator of nuclear factor kappa-b (RANK), receptor activator of nuclear factor-kappa B ligand ( RANKL ) and osteoprotegerin (OPG), and persistent apical periodontitis (PAP). Methods: Patients with pulp necrosis and apical periodontitis at the time of non-surgical root canal treatment were followed up for at least one year. A total of 423 subjects were included, 172 with signs/symptoms of PAP and 251 with apical periodontitis healed. DNA was extracted from saliva and used for genotyping polymorphisms in SOCS1 (rs243327 and rs33977706), TNF-alpha (rs1800629), TNFRSF1A (rs1800693), TNFRSF1B (rs1061622), RANK (rs1805034), RANKL (rs1054016) and OPG (rs1032128) by real-time PCR. The frequency of genotypes and alleles was assessed using chi-squared test or Fisher's exact test and odds ratio. Interactions were also tested using multifactor dimensionality reduction (alpha = 5 %). Results: In the polymorphism rs1800629 in TNF-alpha, carrying at least one A risk allele significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.28-0.79; p =0.004). For the polymorphism rs1054016 in RANKL carrying both T risk alleles significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.24-0.92; p =0.027). None of the other polymorphisms evaluated were associated with PAP (p>0.05). A strong interaction was observed among rs1800629, rs1061622 and rs1054016. Conclusions: Genetic polymorphisms rs1800629 ( TNF-alpha) and rs1054016 ( RANKL) had a strong interaction and were associated with a lower risk to develop PAP. (AU) | |
| Processo FAPESP: | 18/21130-3 - Aspectos moleculares da resposta do hospedeiro na periodontite apical persistente |
| Beneficiário: | Manoel Damiao de Sousa Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |