| Texto completo | |
| Autor(es): |
Almeida-Junior, Luciano Aparecido
;
de Carvalho, Marcio Santos
;
Almeida, Lana Kei Yamamoto
;
Silva-Sousa, Alice Correa
;
Sousa-Neto, Manoel Damiao
;
Silva, Raquel Assed Bezerra
;
Silva, Lea Assed Bezerra
;
Paula-Silva, Francisco Wanderley Garcia
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF ENDODONTICS; v. 49, n. 10, p. 12-pg., 2023-09-28. |
| Resumo | |
Introduction: The aim of this study was to investigate the role of the proinflammatory axis TNF-a-TNFR1 in experimentally induced periapical inflammation and bone resorption in mice. Methods: After receiving Ethics Committee Approval (2019.1.139.58.0), experimental apical periodontitis was induced by means of inoculating oral microorganisms into the root canals of molars of mice. Genetically deficient tumor necrosis factor -a receptor-1 mice (TNFR1(-/-); n = 50) response was compared with that of C57Bl6 wild-type mice (wild-type; n = 50) after 7, 14, 28, and 42 days. The analyses performed were micro-computed tomographic, his-topathologic, histomicrobiological, and histometric evaluation, tartrate-resistant acid phos-phatase staining, immunohistochemistry, and quantitative reverse transcriptase polymerase chain reaction. Data were analyzed by using one-way analysis of variance, followed by Tukey or Bonferroni tests (a = 5%). Results: TNFR1(-/-)mice exhibited lower recruitment of neutrophils at 14, 28, and 42 days (P < .05), which resulted in reduced area and volume of apical periodontitis at 42 days (P < .05). The number of osteoclasts was also lower in TNFR1(-/-)animals at 14 and 42 days (P < .01), along with reduced synthesis of CTSK, MMP-9, and COX-2. Expression of RANKL, but not OPG, was reduced at 14 and 42 days (P < .001). The highest RANKL expression over OPG (ratio > 1) was found in wild-type animals at 7 (P < .0001) and 42 days (P < .001). Conclusions: Periapical inflammation and bone resorption were exacerbated in wild-type animals compared with TNFR1(-/-)mice, demon-strating that the TNF-a-TNFR1 signaling pathway mediated catabolic events in bone after root canal contamination. (J Endod 2023;49:1319-1328.) (AU) | |
| Processo FAPESP: | 19/02432-1 - Papel do fator de necrose tumoral-alfa na inflamação e no reparo pulpar e periapical |
| Beneficiário: | Luciano Aparecido de Almeida Junior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/00204-1 - Papel do fator de necrose tumoral-alfa na inflamação e no reparo pulpar e periapical |
| Beneficiário: | Francisco Wanderley Garcia de Paula e Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |