Advanced Extraction Techniques Combined with Natural Deep Eutectic Solvents for Extracting Phenolic Compounds from Pomegranate (Punica granatum L.) Peels

Autor(es)

de Oliveira, Isadora Lopes ; Dominguez-Rodriguez, Gloria ; Montero, Lidia ; Vigano, Juliane ; Cifuentes, Alejandro ; Rostagno, Mauricio Arial ; Ibanez, Elena

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 25, n. 18, p. 16-pg., 2024-09-01.

Resumo

Pomegranate (Punica granatum L.) peel is a potential source of bioactive phenolic compounds such as ellagic acid and alpha- and beta-punicalagin. This work explores the efficiency of natural deep eutectic solvents combined with ultrasound-assisted extraction (UAE) and pressurized liquid extraction (PLE) for their extraction. Five NaDESs were evaluated by employing UAE (25 degrees C, for 50 min) to determine their total phenolic content (Folin-Ciocalteu assay) and ellagic acid and alpha- and beta-punicalagin contents (high-performance liquid chromatography (HPLC-DAD)). The NaDES composed of choline chloride (ChCl) and glycerol (Gly) (1:2, molar ratio) was the most efficient in the UAE when compared with the rest of the NaDESs and water extracts. Therefore, ChCl:Gly was further evaluated using PLE at different temperatures (40, 80, 120 and 160 degrees C). The PLE-NaDES extract obtained at 80 degrees C for 20 min at 1500 psi exhibited the highest contents of ellagic acid and alpha- and beta-punicalagin compared to the rest of the temperatures and PLE-water extracts obtained under the same extraction conditions. Combining UAE or PLE with a NaDES emerges as a sustainable alternative for extracting ellagic acid and alpha- and beta-punicalagin from pomegranate peel. (AU)

• Processo FAPESP: 22/13565-5 - Desenvolvimento de um método de extração seletivo utilizando solventes alternativos pressurizados para fracionamento de compostos de casca de romã (Punica granatum L.)
• Beneficiário: Isadora Lopes de Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 18/14582-5 - Desenvolvimento de um sistema bidimensional de extração, separação e análise para avaliação da bioatividade de fitoquímicos
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 21/13638-0 - Desenvolvimento de embalagem ativa através da extração de compostos bioativos da casca da romã (Punica granatum L.)
• Beneficiário: Isadora Lopes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado