| Texto completo | |
| Autor(es): |
Sampaio, Caio
;
Cusicanqui Mendez, Daniela Alejandra
;
Buzalaf, Marilia Afonso Rabelo
;
Pessan, Juliano Pelim
;
Cruvinel, Thiago
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Dentistry; v. 145, p. 9-pg., 2024-04-16. |
| Resumo | |
Objective: To assess the effects of arginine, with or without sodium fluoride (NaF; 1,450 ppm), on saliva-derived microcosm biofilms and enamel demineralization. Methods: Saliva-derived biofilms were grown on bovine enamel blocks in 0.2 % sucrose-containing modified McBain medium, according to six experimental groups: control (McBain 0.2 %); 2.5 % arginine; 8 % arginine; NaF; 2.5 % arginine with NaF; and 8 % arginine with NaF. After 5 days of growth, biofilm viability was assessed by colony-forming units counting, laser scanning confocal microscopy was used to determine biofilm vitality and extracellular polysaccharide (EPS) production, while biofilm metabolism was evaluated using the resazurin assay and lactic acid quantification. Demineralization was evaluated by measuring pH in the culture medium and calcium release. Data were analyzed by Kruskal-Wallis' and Dunn's tests (p < 0.05). Results: 8 % arginine with NaF showed the strongest reduction in total streptococci and total microorganism counts, with no significant difference compared to arginine without NaF. Neither 2.5 % arginine alone nor NaF alone significantly reduced microbial counts compared to the control, although in combination, a reduction in all microbial groups was observed. Similar trends were found for biofilm vitality and EPS, and calcium released to the growth medium. Conclusions: 8 % Arginine, with or without NaF, exhibited the strongest antimicrobial activity and reduced enamel calcium loss. Also, NaF enhanced the effects of 2.5 % arginine, yielding similar results to 8 % arginine for most parameters analyzed. Clinical significance: The results provided further evidence on how arginine, with or without NaF, affects oral microcosm biofilms and enamel mineral loss. (AU) | |
| Processo FAPESP: | 18/03866-2 - Efeito da arginina sobre biofilmes dentários e prevenção da desmineralização do esmalte |
| Beneficiário: | Thiago Cruvinel da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/01654-8 - Efeito da aplicação da arginina sobre a composição e metabolismo de biofilmes microcosmos de saliva e prevenção da desmineralização do esmalte |
| Beneficiário: | Daniela Alejandra Cusicanqui Méndez |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |