What are the impacts associated with the replacement of sodium alginate with corn starch on bead formation by jet cutting? The case of Eversa Transform 2.0 immobilization

Autor(es)

Almeida, Francisco Lucas Chaves ; Silveira, Mariana Pereira ; dos Passos, Rafaela Menezes ; da Silva, Thiago Lopes ; Sampaio, Klicia Araujo ; Vieira, Melissa Gurgel Adeodato ; Prata, Ana Silvia ; Forte, Marcus Bruno Soares

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 143, p. 11-pg., 2024-05-07.

Resumo

Entrapment is a strategy widely used for lipase immobilization and recovery. However, mass transfer issues and the high costs of bead production limit the potential of the technique. This study aimed to investigate the impacts associated with the replacement of sodium alginate with corn starch on bead formation by jet cutting. BET analysis showed surface areas of 1.02 and 1.24 m 2 g(-1) for beads containing 25 % (B25) and 50 % (B50) starch, respectively. XPS showed nitrogen distribution rates of 7.40-8.77 % (B25) and 4.75 - 5.10 % (B50). Both immobilized derivatives achieved activity recoveries of 56.23 % +/- 5.73 % (B25) and 51.58 % +/- 5.56 % (B50) and maximum specific activity of 1500 U g(-1) protein. The thermal inactivation constant values of free lipase were 0.0096 and 0.0285 min(-1) at 70 and 75 degrees C, while to immobilized lipases were 7 -fold and 2 -fold lower than that at 70 and 75 degrees C, respectively. The half-life time of free lipase and immobilized derivatives (B25 and B50), at 70 degrees C, were 72.20, 577.62 and 533.19 min, respectively. Biodiesel yields of 50.49 % +/- 5.67 % and 51.16 % +/- 9.91 % were achieved using B25 and B50, respectively. These findings demonstrate lipase can be successfully immobilized on alginate-starch beads by jet cutting. (AU)

• Processo FAPESP: 20/14683-6 - EMU concedido no processo 2014/21252-0: Equipamento de Espectroscopia no Infravermelho FTIR, da marca Agilent, modelo Cary 630
• Beneficiário: Antonio José de Almeida Meirelles
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 19/03399-8 - Imobilização de lipase por técnica de jet cutter e produção de biodiesel usando o biocatalisador em reator tubular
• Beneficiário: Francisco Lucas Chaves Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/21252-0 - Equilíbrio e processos de produção de biocombustíveis e bioprodutos
• Beneficiário: Antonio José de Almeida Meirelles
• Modalidade de apoio: Auxílio à Pesquisa - Temático