Dry blood spots as a sampling strategy to identify insulin resistance markers during a dietary challenge

Autor(es)

Duarte, Stephany Goncalves ; Donado-Pestana, Carlos M. ; More, Tushar H. ; Rodrigues, Larissa ; Hiller, Karsten ; Fiamoncini, Jarlei

• Tipo de documento: Artigo Científico
• Fonte: GENES AND NUTRITION; v. 19, n. 1, p. 9-pg., 2024-12-01.

Resumo

This study aimed to identify markers of postprandial dysglycemia in the blood of self-described healthy individuals using dry blood spots (DBS) as a sampling strategy. A total of 54 volunteers, including 31 women, participated in a dietary challenge. They consumed a high-fat, high-sugar mixed meal and underwent multiple blood sampling over the course of 150 min to track their postprandial responses. Blood glucose levels were monitored with a portable glucometer and individuals were classified into two groups based on the glucose area under the curve (AUC): High-AUC (H-AUC) and Low-AUC (L-AUC). DBS sampling was performed at the same time points as the assessment of glycemia using Whatman 903 Protein Saver filter paper. A gas chromatography-mass spectrometry-based metabolite profiling was conducted in the DBS samples to assess postprandial changes in blood metabolome. Higher concentrations of metabolites associated with insulin resistance were observed in individuals from the H-AUC group, including sugars and sugar-derived products such as fructose and threonic acid, as well as organic acids and fatty acids such as succinate and stearic acid. Several metabolites detected in the GC-MS analysis remained unidentified, indicating that other markers of hyperglycemia remain to be discovered in DBS. Based on these observations, we demonstrated that the use of DBS as a non-invasive and inexpensive sampling tool allows the identification of metabolites markers of dysglycemia in the postprandial period. (AU)

• Processo FAPESP: 21/09237-0 - Metabolismo pós-prandial e inflamação: o papel dos ácidos biliares como moléculas de sinalização
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• Modalidade de apoio: Bolsas no Brasil - Doutorado
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Modalidade de apoio: Auxílio à Pesquisa - Projeto Inicial
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