Electrochemical detection of poly(3-hydroxybutyrate) production from Burkholderia glumae MA13 using a molecularly imprinted polymer-reduced graphene oxide modified electrode

Autor(es)

da Conceicao, Emanuela ; Buffon, Edervaldo ; Beluomini, Maisa Azevedo ; Falone, Max Fabricio ; de Andrade, Fernanda Batista ; Contiero, Jonas ; Stradiotto, Nelson Ramos

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 191, n. 8, p. 12-pg., 2024-08-01.

Resumo

The development and application of an electrochemical sensor is reported for detection of poly(3-hydroxybutyrate) (P3HB) - a bioplastic derived from agro-industrial residues. To overcome the challenges of molecular imprinting of macromolecules such as P3HB, this study employed methanolysis reaction to break down the P3HB biopolymer chains into methyl 3-hydroxybutyrate (M3HB) monomers. Thereafter, M3HB were employed as the target molecules in the construction of molecularly imprinted sensors. The electrochemical device was then prepared by electropolymerizing a molecularly imprinted poly (indole-3-acetic acid) thin film on a glassy carbon electrode surface modified with reduced graphene oxide (GCE/rGO-MIP) in the presence of M3HB. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy with field emission gun (SEM-FEG), Raman spectroscopy, attenuated total reflection Fourier-transform infrared (ATR-FTIR) and X-ray Photoelectron Spectroscopy (XPS) were employed to characterize the electrode surface. Under ideal conditions, the MIP sensor exhibited a wide linear working range of 0.1 - 10 nM and a detection limit of 0.3 pM (n = 3). The sensor showed good repeatability, selectivity, and stability over time. For the sensor application, the bioproduction of P3HB was carried out in a bioreactor containing the Burkholderia glumae MA13 strain and sugarcane byproducts as a supplementary carbon source. The analyses were validated through recovery assays, yielding recovery values between 102 and 104%. These results indicate that this MIP sensor can present advantages in the monitoring of P3HB during the bioconversion process. (AU)

• Processo FAPESP: 17/22401-8 - Fruto-refinaria: processos de obtenção, métodos de caracterização e geração de produtos oriundos de resíduos da fruticultura
• Beneficiário: Nelson Ramos Stradiotto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/12131-6 - Sensores eletroquímicos baseados em eletrodos impressos modificados com nanoporos metálicos e monitorados por rede sem fio para determinação de flavonóides nos resíduos da indústria cítrica
• Beneficiário: Maísa Azevedo Beluomini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/13818-8 - Desenvolvimento de sensores descartáveis baseados em óxido de grafeno 3D, nanopartículas metálicas e polímeros molecularmente impressos para determinação de ácidos fenólicos em resíduos da fruticultura
• Beneficiário: Edervaldo Buffon
• Modalidade de apoio: Bolsas no Brasil - Doutorado