CSC^highE-cadherin^low immunohistochemistry panel predicts poor prognosis in oral squamous cell carcinoma

Autor(es)

Ortiz, Rafael Carneiro ; Amor, Nadia Ghinelli ; Saito, Luciana Mieli ; Santesso, Mariana Rodrigues ; Lopes, Nathalia Martins ; Buzo, Rodrigo Fonseca ; Fonseca, Angelica Cristina ; Amaral-Silva, Gleyson Kleber ; Moyses, Raquel Ajub ; Rodini, Camila Oliveira

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 14, n. 1, p. 13-pg., 2024-05-08.

Resumo

Identifying marker combinations for robust prognostic validation in primary tumour compartments remains challenging. We aimed to assess the prognostic significance of CSC markers (ALDH1, CD44, p75NTR, BMI-1) and E-cadherin biomarkers in OSCC. We analysed 94 primary OSCC and 67 metastatic lymph node samples, including central and invasive tumour fronts (ITF), along with clinicopathological data. We observed an increase in ALDH1(+)/CD44(+)/BMI-1(-) tumour cells in metastatic lesions compared to primary tumours. Multivariate analysis highlighted that elevated p75NTR levels (at ITF) and reduced E-cadherin expression (at the tumour centre) independently predicted metastasis, whilst ALDH1(high) exhibited independent predictive lower survival at the ITF, surpassing the efficacy of traditional tumour staging. Then, specifically at the ITF, profiles characterized by (CSCE)-E-high-cadherin(low) (ALDH1(high)p75NTR(high)E-cadherin(low)) and (CSCE)-E-intermediate-cadherin(low) (ALDH1 or p75NTR(high)E-cadherin(low)) were significantly associated with worsened overall survival and increased likelihood of metastasis in OSCC patients. In summary, our study revealed diverse tumour cell profiles in OSCC tissues, with varying CSC and E-cadherin marker patterns across primary tumours and metastatic sites. Given the pivotal role of reduced survival rates as an indicator of unfavourable prognosis, the immunohistochemistry profile identified as (CSCE)-E-high-cadherin(low) at the ITF of primary tumours, emerges as a preferred prognostic marker closely linked to adverse outcomes in OSCC. (AU)

• Processo FAPESP: 13/07245-9 - Investigação do papel das células-tronco de câncer e do microambiente no processo de transição epitélio-mesenquimal, invasão e metástase do carcinoma epidermóide de boca
• Beneficiário: Camila de Oliveira Rodini Pegoraro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/06945-2 - Análise imuno-histoquímica diferencial e comparativa entre CEC de boca primário e lesões metastáticas correspondentes
• Beneficiário: Rafael Carneiro Ortiz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/53839-2 - Criação do Laboratório de Patologia Digital através do uso do escaneador de lâminas histológicas (Aperio® Scanscope CS)
• Beneficiário: Oslei Paes de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 17/25022-8 - Investigação da presença de células amebóides em Carcinoma Epidermóide de Boca e sua participação no processo de metástase
• Beneficiário: Rafael Carneiro Ortiz
• Modalidade de apoio: Bolsas no Brasil - Doutorado