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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential effects of female sex hormones on cellular recruitment and tracheal reactivity after formaldehyde exposure

Texto completo
Autor(es):
Lino-dos-Santos-Franco, Adriana ; Amemiya, Renata Midori ; Ligeiro de Oliveira, Ana Paula [1] ; Breithaupt-Faloppa, Ana Cristina ; Damazo, Amilcar Sabino [2] ; Oliveira-Filho, Ricardo Martins ; Tavares-de-Lima, Wothan [3]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508900 Sao Paulo - Brazil
[2] Fed Univ Cuiaba, Fac Med Sci, Dept Basic Sci Hlth, Cuiaba - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Lab Fisiopatol Inflamacao Expt, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Toxicology Letters; v. 205, n. 3, p. 327-335, SEP 10 2011.
Citações Web of Science: 3
Resumo

Female sex hormones (FSHs) exert profound regulatory effects on the course of lung inflammation due to allergic and non-allergic immune responses. As pollution is one of the pivotal factors to induce lung dysfunction, in this study we investigated the modulatory role of FSHs on lung inflammation after a formaldehyde (FA) exposure. For this purpose, lung and systemic inflammatory responses were evaluated in terms of leukocytes countings in bronchoalveolar lavage (BAL), peripheral blood and bone marrow lavage from 7-day ovariectomized (OVx) and Sham-OVx rats subjected to FA inhalation for 3 consecutive days. The hypothesized link between effects of FSHs on expression of adhesion molecules and mast cells degranulation was also studied. Once exposed to FA, Sham-OVx rats increased the number of total cells recovered in BAL and of leukocytes in peripheral blood, and decreased the counts in bone marrow. By contrast, in OVx rats upon FA exposure there was a reduction of the total cells counts in BAL and of blood leukocytes: lung expressions of ICAM-1 and Mac-1 were depressed, but the number of bone marrow cells did not vary. Estradiol treatment of OVx rats increased the total cells in BAL and decreased the number of blood leukocytes, whereas the number of bone marrow cell remained unaltered. Progesterone treatment, in turn increased the total cells in BAL and blood leukocytes, but decreased the number of bone marrow cells. OVx rats exposed to FA developed tracheal hyperresponsiveness to methacholine (MCh). A similarly altered response was found between the tracheal segments of Sham-OVx rats after FA exposure and that found in tracheae of naive rats. Estradiol treatment prevented FA-induced tracheal hyperresponsiveness to MCh whereas progesterone was ineffective in this regard. In addition, OVx rats upon FA exposure significantly increased both, the ability of mast cell degranulation and serum corticosterone levels. In conclusion, it was found that FSHs act by distinct control mechanisms on FA-induced lung inflammation and tracheal hyperresponsiveness, since at low circulating levels of FSHs (such as those after OVx) there is some resistance to the development of a lung inflammatory response, but the cholinergic tracheal responsiveness is exacerbated. Our data also help to understand the involvement of FSHs on mast cells activity after pollutants exposure and add information regarding the role of FSHs on the mechanisms related to endothelium-leukocyte interactions. (C) 2011 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 08/58108-3 - Efeitos da exposicao ao formaldeido sobre a inflamacao alergica pulmonar: papel modulador dos hormonios sexuais femininos
Beneficiário:Renata Midori Amemiya
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/50766-1 - Repercussao pulmonar e sistemica apos exposicao de ratos ao formaldeido: papel regulador das especies reativas derivadas do oxigenio e do nitrogenio.
Beneficiário:Adriana Lino dos Santos Franco
Linha de fomento: Bolsas no Brasil - Pós-Doutorado