| Texto completo | |
| Autor(es): |
de Almeida, Otavio Guilherme Goncalves
;
Bertozzi, Bruno Gerfi
;
Rocha, Liliana de Oliveira
;
von Hertwig, Aline Morgan
;
Arroyo, Diana Mara Dias
;
de Martinis, Elaine Cristina Pereira
;
Nascimento, Maristela Silva
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Food Microbiology; v. 420, p. 10-pg., 2024-05-31. |
| Resumo | |
Peanut-based products have been associated with Salmonella foodborne outbreaks and/or recalls worldwide. The ability of Salmonella to persist for a long time in a low moisture environment can contribute to this kind of contamination. The objective of this study was to analyse the genome of five S. enterica enterica strains isolated from the peanut supply chain in Brazil, as well as to identify genetic determinants for survival under desiccation and validate these findings by phenotypic test of desiccation stress. The strains were in silico serotyped using the platform SeqSero2 as Miami (M2851), Javiana (M2973), Oranienburg (M2976), Muenster (M624), and Glostrup/Chomedey (M7864); with phylogenomic analysis support. Based on Multilocus Sequence Typing (MLST) the strains were assigned to STs 140, 1674, 321, 174, and 2519. In addition, eight pathogenicity islands were found in all the genomes using the SPIFinder 2.0 (SPI-1, SPI-2, SPI-3, SPI-5, SPI-9, SPI-13, SPI-14). The absence of a SPI-4 may indicate a loss of this island in the surveyed genomes. For the pangenomic analysis, 49 S. enterica genomes were input into the Roary pipeline. The majority of the stress related genes were considered as soft-core genes and were located on the chromosome. A desiccation stress phenotypic test was performed in trypticase soy broth (TSB) with four different water activity (a(w)) values. M2976 and M7864, both isolated from the peanut samples with the lowest a(w), showed the highest OD570nm in TSB a(w) 0.964 and were statistically different (p < 0.05) from the strain isolated from the peanut sample with the highest a(w) (0.997). In conclusion, genome analyses have revealed signatures of desiccation adaptation in Salmonella strains, but phenotypic analyses suggested the environment influences the adaptive ability of Salmonella to overcome desiccation stress. (AU) | |
| Processo FAPESP: | 17/13759-6 - Prospecção metagenômica de bactérias relacionadas a quorum sensing durante a fermentação espontânea do cacau |
| Beneficiário: | Otávio Guilherme Gonçalves de Almeida |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 21/06809-2 - Adesão a seco de Salmonella spp. e Cronobacter spp. em superfícies e resistência a processos de higienização |
| Beneficiário: | Maristela da Silva Do Nascimento |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |