Combined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model

Autor(es)

Ribeiro, Rafael Antonio de Oliveira ; Peruchi, Victoria ; Sores, Igor Paulino Mendes ; Mon, Filipe Koon Wu ; Soares, Diana Gabriela ; Hebling, Josimeri ; Costa, Carlos Alberto de Souza

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 28, n. 12, p. 13-pg., 2024-11-29.

Resumo

Objectives To evaluate the feasibility of using a 3D model with human dental pulp cells (HDPCs) to compare bleaching therapies and assess whether coating enamel with a nanofiber scaffold (NS) and polymeric catalyst primer (PCP), combined with violet LED (LEDv) irradiation, enhances bleaching efficacy (BE) and reduces cytotoxicity (CT). Materials and methods After using NS + PCP to cover enamel of enamel/dentin discs adapted to artificial pulp chambers containing 3D culture with HDPCs, a bleaching gel with 35%H2O2 was applied and then irradiated with LEDv. The following groups were established (n = 8): NC - no treatment; PC- 35%H2O2 for 45 min, and EXP: NS + PCP + 35%H2O2 + LEDv for 15 min. The study evaluated BE (Delta E-00 and Delta WID), CT (alamarBlue), and HDPCs gene modulation (TNF, IL1B, PTGS2, IL8, IL6, PPRAG, HMOX1, DSPP, DMP1, SPP1, BGLAP, and ALPL; RTqPCR). Results BE showed no significant difference between PC and EXP (p > 0.05). EXP had lower oxidative stress, higher cell viability, reduced inflammatory marker expression, and increased mineralization marker expression compared to PC (p < 0.05). Conclusion The 3D model using HDPCs effectively compared bleaching protocols. Coating enamel with NS + PCP and applying violet LED (LEDv) reduced bleaching time from 45 to 15 min while lowering cytotoxicity compared to conventional in-office treatments. Clinical relevance This study shows that a 3D model with human dental pulp cells (HDPCs) effectively compares tooth bleaching protocols. The combination of a nanofiber scaffold with violet LED (LEDv) reduces bleaching time, as well as minimizes cytotoxicity and inflammation, offering a safer alternative to conventional treatments. (AU)

• Processo FAPESP: 23/02780-5 - Desenvolvimento e aplicação de um primer polimérico contendo TiF4 para clareamento dental de consultório associado a irradiação com LED violeta
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/01184-4 - Desenvolvimento de estratégias inovadoras para clareamento dental de consultório: eficácia estética e biocompatibilidade associadas à redução do tempo clínico
• Beneficiário: Rafael Antonio de Oliveira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado