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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Serotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapnia

Texto completo
da Silva, Glauber S. F. [1] ; Giusti, Humberto [1] ; Benedetti, Mauricio [1] ; Dias, Mirela B. [2] ; Gargaglioni, Luciane H. [3] ; Branco, Luiz Guilherme S. [4] ; Glass, Mogens L. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo USP FMRP, Dept Physiol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Fed Goias, Inst Biol Sci, Dept Physiol Sci, Goiania, Go - Brazil
[3] Sao Paulo State Univ UNESP FCAV Jaboticabal, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[4] Univ Sao Paulo USP FORP, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY; v. 462, n. 3, p. 407-418, SEP 2011.
Citações Web of Science: 30

Serotonergic (5-HT) neurons in the nucleus raphe obscurus (ROb) are involved in the respiratory control network. However, it is not known whether ROb 5-HT neurons play a role in the functional interdependence between central and peripheral chemoreceptors. Therefore, we investigated the role of ROb 5-HT neurons in the ventilatory responses to CO(2) and their putative involvement in the central-peripheral CO(2) chemoreceptor interaction in unanaesthetised rats. We used a chemical lesion specific for 5-HT neurons (anti-SERT-SAP) of the ROb in animals with the carotid body (CB) intact or removed (CBR). Pulmonary ventilation (V (E)), body temperature and the arterial blood gases were measured before, during and after a hypercapnic challenge (7% CO(2)). The lesion of ROb 5-HT neurons alone (CB intact) or the lesion of 5-HT neurons of ROb+CBR did not affect baseline V (E) during normocapnic condition. Killing ROb 5-HT neurons (CB intact) significantly decreased the ventilatory response to hypercapnia (p < 0.05). The reduction in CO(2) sensitivity was approximately 15%. When ROb 5-HT neurons lesion was combined with CBR (anti-SERT-SAP+CBR), the V (E) response to hypercapnia was further decreased (-31.2%) compared to the control group. The attenuation of CO(2) sensitivity was approximately 30%, and it was more pronounced than the sum of the individual effects of central (ROb lesion; -12.3%) or peripheral (CBR; -5.5%) treatments. Our data indicate that ROb 5-HT neurons play an important role in the CO(2) drive to breathing and may act as an important element in the central-peripheral chemoreception interaction to CO(2) responsiveness. (AU)

Processo FAPESP: 07/51581-2 - Papel do núcleo obscuro da rafe na quimiossensibilidade ao CO2/pH
Beneficiário:Mogens Lesner Glass
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/60696-5 - Papel do núcleo obscuro da rafe na quimiossensibilidade ao CO2/pH
Beneficiário:Glauber dos Santos Ferreira da Silva
Linha de fomento: Bolsas no Brasil - Doutorado