Disposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2

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Rocha, Danielly S. ; Baldo, Thaisa A. ; Silva-Neto, Habdias A. ; Duarte-Junior, Gerson F. ; Bazilio, Gabriela S. ; Borges, Clayton L. ; Parente-Rocha, Juliana A. ; de Araujo, William R. ; de Siervo, Abner ; Paixa, Thiago L. R. C. ; Coltro, Wendell K. T.

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 268, p. 9-pg., 2023-10-29.

Resumo

This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protec-tive layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)6]3-/4-as a redox probe. The method presented a linear response in the concentration range from 250 pg mL-1 to 20 mu g mL-1 S protein, with a limit of detection of 36.3 pg mL-1. The proposed immunosensor was stable for up to two weeks when stored at 4 degrees C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak. (AU)

• Processo FAPESP: 07/54829-5 - Estrutura eletrônica e geométrica de nano materiais: um estudo por radiação sincroton
• Beneficiário: Richard Landers
• Modalidade de apoio: Auxílio à Pesquisa - Temático
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• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/08244-5 - Estudo das propriedades magnéticas de filmes ultrafinos e nanopartículas de Pd e PdAu via XMCD
• Beneficiário: Abner de Siervo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais
• Beneficiário: William Reis de Araujo
• Modalidade de apoio: Auxílio à Pesquisa - Projeto Inicial