Phylogenetic Analysis of Hepatitis B Virus Genotype F Complete Genome Sequences From Chilean Patients With Chronic Infection

Autor(es)

Venegas, Mauricio ^[1] ; Alvarado-Mora, Monica V. ^{[2, 3]} ; Villanueva, Rodrigo A. ^[4] ; Rebello Pinho, Joao R. ^{[2, 3]} ; Carrilho, Flair J. ^{[2, 3]} ; Locarnini, Stephen ^[5] ; Yuen, Lilly ^[5] ; Brahm, Javier ^[1]

Afiliação do(s) autor(es)

^[1] Univ Chile Clin Hosp, Dept Med, Gastroenterol Sect, Santiago - Chile

^[2] Univ Sao Paulo, Sao Paulo Inst Trop Med, Lab Trop Gastroenterol & Hepatol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Gastroenterol, Sch Med, Sao Paulo - Brazil

^[4] Fac Med, Inst Biomed Sci, Virol Program, Lab Hepatitis Viruses, Santiago - Chile

^[5] Victorian Infect Dis Reference Lab, Melbourne, Vic - Australia

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 83, n. 9, p. 1530-1536, SEP 2011.
• Citações Web of Science: 15

Resumo

Molecular epidemiological data concerning the hepatitis B virus (HBV) in Chile are not known completely. Since the HBV genotype F is the most prevalent in the country, the goal of this study was to obtain full HBV genome sequences from patients infected chronically in order to determine their subgenotypes and the occurrence of resistance-associated mutations. Twenty-one serum samples from antiviral drug-naive patients with chronic hepatitis B were subjected to full-length PCR amplification, and both strands of the whole genomes were fully sequenced. Phylogenetic analyses were performed along with reference sequences available from GenBank (n = 290). The sequences were aligned using Clustal X and edited in the SE-AL software. Bayesian phylogenetic analyses were conducted by Markov Chain Monte Carlo simulations (MCMC) for 10 million generations in order to obtain the substitution tree using BEAST. The sequences were also analyzed for the presence of primary drug resistance mutations using CodonCode Aligner Software. The phylogenetic analyses indicated that all sequences were found to be the HBV subgenotype F1b, clustered into four different groups, suggesting that diverse lineages of this subgenotype may be circulating within this population of Chilean patients. J. Med. Virol. 83: 1530-1536, 2011. (C) 2011 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular