Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins

Autor(es)

Bermudez-Puga, Sebastain ; Dias, Meriellen ; Reis, Iara Lima ; de Oliveira, Taciana Freire ; de Almeida, Sonia Regina Yokomizo ; Mendes, Maria Anita ; Moore, Simon J. ; Almeida, Jose R. ; Proano-Bolanos, Carolina ; de Souza Oliveira, Ricardo Pinheiro

• Tipo de documento: Artigo Científico
• Fonte: Biochimie; v. 225, p. 8-pg., 2024-06-06.

Resumo

Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15] CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 mM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination. (c) 2024 Elsevier B.V. and Soci & eacute;t & eacute; Fran & ccedil;aise de Biochimie et Biologie Mol & eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU)

• Processo FAPESP: 18/25511-1 - Bioprospecção de bactérias probióticas bacteriocinogênicas: da otimização do cultivo à aplicação em sistemas de produção animal
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/15599-9 - EMU concedido no processo 2018/25511-1: Sistema de Cromatografia Líquida de Alta Pressão Semi-Preparativo
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 22/02936-2 - Probióticos e bacteriocinas na alimentação da Tilápia-do-Nilo: efeitos no desempenho zootécnico de crescimento, imunologia e infecção experimental
• Beneficiário: Sebastián Armando Bermúdez Puga
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto