| Texto completo | |
| Autor(es): |
Kopel, Beatriz
;
Serino-Silva, Caroline
;
Jantsch, Rebeca Barcelos
;
Sorila, Igor Castellar
;
Sant'Anna, Savio S.
;
Grego, Kathleen Fernandes
;
Tanaka-Azevedo, Anita Mitico
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 252, p. 9-pg., 2024-12-01. |
| Resumo | |
Naja kaouthia is a medically important snake, widely distributed throughout Southeast Asia, with a diverse venom composition. N. kaouthia venom is subject to significant intraspecific variation, caused by several factors, such as the wide geographic distribution of the species, sexual and ontogenetic factors. However, individual variation is a factor that has only been studied with small sample size groups and/or with pooled samples. With this in mind, this study evaluates the composition and in-vitro enzymatic activities of 29 individual venom samples from specimens born in captivity, with a similar genetic background caused by inbreeding, using SDSPAGE under reducing conditions, RP-HPLC profiles and enzymatic activities of PLA2, LAAO and proteolytic activity over azocasein. Even in this scenario, we were able to observe significant variations in abundance and activity of PLA2. Individual variations in LAAO activity, as well as a sexual dimorphism in which males present a significantly higher LAAO activity than females were observed. Phosphodiasterase and CRiSP abundance were also found and considered to have multiple effects in the clinical manifestations of envenomation by presenting synergistic effects with other proteins from N. kaouthia venom. The RP-HPLC profiles were better at detecting compositional differences than SDS-PAGE profiles and better correlated with enzymatic activities, being a better technique to screen variation profiles and reinforcing the importance of individual venom analysis prior to pooling. (AU) | |
| Processo FAPESP: | 21/11707-4 - Avaliação de métodos alternativos in vitro à utilização de animais para os ensaios in vivo no estudo de venenos de serpentes: princípio dos 3R's |
| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 24/02642-4 - Perfil ontogenético do veneno e potencial de bioprospecção das toxinas three-finger da serpente Naja kaouthia |
| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/13137-3 - Análise da variação geográfica (insular e continental) do veneno de serpentes do gênero Bothrops de maior importância médica na região Norte brasileira: um problema de saúde pública e de educação ambiental |
| Beneficiário: | Kathleen Fernandes Grego |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/13820-5 - Estudo da composição e atividades bioquímicas do veneno das serpentes senis do gênero Crotalus e isolamento e purificação da PLA2 desses venenos |
| Beneficiário: | Igor Castellar Sorrila |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 21/05405-5 - Análise da composição e neutralização do veneno de Naja kaouthia: um estudo para o aprimoramento do soro antielapídico |
| Beneficiário: | Beatriz Kopel |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |