Collagen-Binding Nanoparticles for Paclitaxel Encapsulation and Breast Cancer Treatment

Autor(es)

Passos, Julia Sapienza ; Lopes, Luciana B. ; Panitch, Alyssa

• Tipo de documento: Artigo Científico
• Fonte: ACS BIOMATERIALS SCIENCE & ENGINEERING; v. 9, n. 12, p. 16-pg., 2023-11-20.

Resumo

In this study, we developed a novel hybrid collagen-binding nanocarrier for potential intraductal administration and local breast cancer treatment. The particles were formed by the encapsulation of nanostructured lipid carriers (NLCs) containing the cytotoxic drug paclitaxel within a shell of poly(N-isopropylacrylamide) (pNIPAM), and were functionalized with SILY, a peptide that binds to collagen type I (which is overexpressed in the mammary tumor microenvironment) to improve local retention and selectivity. The encapsulation of the NLCs in the pNIPAM shell increased nanoparticle size by approximately 140 nm, and after purification, a homogeneous system of hybrid nanoparticles (similar to 96%) was obtained. The nanoparticles exhibited high loading efficiency (<76%) and were capable of prolonging paclitaxel release for up to 120 h. SILY-modified nanoparticles showed the ability to bind to collagen-coated surfaces and naturally elaborated collagen. Hybrid nanoparticles presented cytotoxicity up to 3.7-fold higher than pNIPAM-only nanoparticles on mammary tumor cells cultured in monolayers. In spheroids, the increase in cytotoxicity was up to 1.8-fold. Compared to lipid nanoparticles, the hybrid nanoparticle modified with SILY increased the viability of nontumor breast cells by up to 1.59-fold in a coculture model, suggesting the effectiveness and safety of the system. (AU)

• Processo FAPESP: 18/13877-1 - Nanocarreadores para a quimioprevenção e tratamento localizado de tumores de mama
• Beneficiário: Luciana Biagini Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 21/12664-7 - Síntese de carreador lipídico nanoestruturado termorresponsivo com revestimento de poli(N-isopropilacrilamida) para encapsulação de fármacos e administração intraductal no tecido mamário
• Beneficiário: Julia Sapienza Passos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 20/01208-8 - Sistemas nanoestruturados bioadesivos para administração intraductal e tratamento localizado do Câncer de Mama
• Beneficiário: Julia Sapienza Passos
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto