Ripe papaya pectins inhibit the proliferation of colon cancer spheroids and the formation of chemically induced aberrant crypts in rats colons

Autor(es)

Donadio, Janaina L. S. ; do Prado, Samira Bernardino Ramos ; Soares, Caroline Giacomelli ; Tamarossi, Rodrigo Invernort ; Heidor, Renato ; Moreno, Fernando Salvador ; Fabi, Joao Paulo

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 331, p. 15-pg., 2024-02-02.

Resumo

Pectins are a class of soluble polysaccharides that can have anticancer properties through several mechanisms. This study aimed to characterize the molecular structure of water-soluble fractions (WSF) derived from ripe and unripe papayas and assess their biological effects in two models: the 3D colon cancer spheroids to measure cell viability and cytotoxicity, and the in vivo model to investigate the inhibition of preneoplastic lesions in rats. WSF yield was slightly higher in ripe papaya, and both samples mainly consisted of pectin. Both pectins inhibited the growth of colon cancer HT29 and HCT116 spheroids. Unripe pectin disturbed HT29/NIH3T3 spheroid formation, decreased HCT116 spheroid viability, and increased spheroid cytotoxicity. Ripe pectin had a more substantial effect on the reduction of spheroid viability for HT29 spheroids. Furthermore, in vivo experiments on a rat model revealed a decrease in aberrant crypt foci (ACF) formation for both pectins and increased apoptosis in colonocytes for ripe papaya pectins. The results suggest potential anticancer properties of papaya pectin, with ripe pectin showing a higher potency. (AU)

• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 22/12834-2 - Efeitos das pectinas modificadas na saúde intestinal: estudos pré-clínicos e clínicos para avaliar efeitos celulares, transcricionais e epigenéticos
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/07292-3 - Influência de pectinas do mamão papaia na resposta inflamatória em modelos 3D da mucosa colorretal humana
• Beneficiário: Janaina Lombello Santos Donadio
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado