Insights on the genomic diversity, virulence and resistance profile of a Campylobacter jejuni strain isolated from a hospitalized patient in Brazil

Autor(es)

Gomes, Carolina Nogueira ; Frazao, Miliane Rodrigues ; Seribelli, Amanda Aparecida ; Barker, Dillon Oliver Reese ; Che, Emily Victoria ; Nogueira, Mara Correa Lelles ; Taboada, Eduardo Napoleon ; Falcao, Juliana Pfrimer

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 55, n. 2, p. 11-pg., 2024-03-28.

Resumo

Campylobacteriosis is currently recognized as one of the major causes of foodborne bacterial diseases worldwide. In Brazil, there is insufficient data to estimate the impact of Campylobacter in public health. The aim of this present study was to characterize a C. jejuni CJ-HBSJRP strain isolated from a hospitalized patient in Brazil by its ability to invade human Caco-2 epithelial cells, to survive in U937 human macrophages, and to assess its phenotypic antimicrobial resistance profile. In addition, prophages, virulence and antimicrobial resistance genes were search using whole-genome sequencing data. The genetic relatedness was evaluated by MLST and cgMLST analysis by comparison with 29 other C. jejuni genomes isolated from several countries. The CJ-HBSJRP strain showed an invasion percentage of 50% in Caco-2 polarized cells, 37.5% of survivability in U937 cells and was phenotypically resistant to ampicillin, ciprofloxacin and nalidixic acid. A total of 94 virulence genes related to adherence, biofilm, chemotaxis, immune modulation, invasion process, metabolism, motility and toxin were detected. The resistance genes bla(OXA-605) (bla(OXA-61)), cmeB and mutations in the QRDR region of gyrA were also found and none prophages were detected. The MLST analysis showed 23 different STs among the strains studied. Regarding cgMLST analysis, the CJ-HBSJRP strain was genetically distinct and did not group closely to any other isolate. The results obtained reinforce the pathogenic potential of the CJHBSJRP strain and highlighted the need for more careful attention to Campylobacter spp. infections in Brazil since this pathogen has been the most commonly reported zoonosis in several countries worldwide. (AU)

• Processo FAPESP: 19/19338-8 - Genômica, transcriptômica e caracterização fenotipica de linhagens de Campylobacter jejuni isoladas de diversas fontes durante 20 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/23408-0 - Análise fenotípica, sequenciamento do genoma e transcriptoma de linhagens de Campylobacter coli isoladas de diversas fontes durante 16 anos no Brasil
• Beneficiário: Carolina Nogueira Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/26043-1 - Sequenciamento do genoma completo e análise genômica comparativa de linhagens de Campylobacter coli isoladas de diversas fontes durante 16 anos no Brasil
• Beneficiário: Carolina Nogueira Gomes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 22/07013-0 - Análises do genoma, transcriptoma, construção de mutantes e caracterização fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes durante 25 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular