| Texto completo | |
| Autor(es): |
Santana, Bianca de Melo
;
Armentano, Giovana Marchini
;
Ferreira, Dayana Agnes Santos
;
de Freitas, Camila Simoes
;
Carneiro-Ramos, Marcela Sorelli
;
Seabra, Amedea Barozzi
;
Christodoulides, Myron
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS APPLIED MATERIALS & INTERFACES; v. 16, n. 17, p. 10-pg., 2024-04-18. |
| Resumo | |
Resistance to antibiotics and antimicrobial compounds is a significant problem for human and animal health globally. The development and introduction of new antimicrobial compounds are urgently needed, and copper oxide nanoparticles (CuO NPs) have found widespread application across various sectors including biomedicine, pharmacy, catalysis, cosmetics, and many others. What makes them particularly attractive is the possibility of their synthesis through biogenic routes. In this study, we synthesized biogenic green tea (GT, Camellia sinensis)-derived CuO NPs (GT CuO NPs) and examined their biophysical properties, in vitro toxicity for mammalian cells in culture, and then tested them against Neisseria gonorrhoeae, an exemplar Gram-negative bacterium from the World Health Organization's Priority Pathogen List. We compared our synthesized GT CuOP NPs with commercial CuO NPs (Com CuO NPs). Com CuO NPs were significantly more cytotoxic to mammalian cells (IC50 of 7.32 mu g/mL) than GT CuO NPs (IC50 of 106.1 mu g/mL). GT CuO NPs showed no significant increase in bax, bcl2, il6, and il1 beta mRNA expression from mammalian cells, whereas there were notable rises after treatment with Com CuO NPs. GT-CuO NPs required concentrations of 0.625 and 3.125 mu g/mL to kill 50 and 100% of bacteria, respectively, whereas Com-CuO NPs needed concentrations of 15.625 and 30 mu g/mL to kill 50 and 100% of bacteria, and the antibiotic ceftriaxone killed 50 and 100% with 3.125 and 30 mu g/mL. Gonococci could be killed within 30 min of exposure to GT CuO NPs and the NPs could kill up to 10(7) within 1 h. In summary, this is the first report to our knowledge that describes the bioactivity of biogenic CuO NPs against N. gonorrhoeae. Our data suggest that biogenic nanoparticle synthesis has significant advantages over traditional chemical routes of synthesis and highlights the potential of GT-CuO NPs in addressing the challenges posed by multidrug-resistant Neisseria gonorrhoeae infections. (AU) | |
| Processo FAPESP: | 21/13821-9 - Avaliação de propriedades antimicrobianas de nano/biomateriais liberadores de NO para o combate da Neisseria gonorrhoeae |
| Beneficiário: | Bianca de Melo Santana |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica |
| Processo FAPESP: | 22/00153-0 - Biofotônica aplicada ao estudo de vesículas extracelulares como conectores da conversa rim-coração |
| Beneficiário: | Marcela Sorelli Carneiro Ramos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/08566-7 - Desenvolvimento de nanopartículas magnéticas de Fe3O4@SiO2 contendo doadores de óxido nítrico para aplicações biomédicas |
| Beneficiário: | Bianca de Melo Santana |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 22/00321-0 - Nanomateriais aliados a doadores de óxido nítrico para aplicações biológicas |
| Beneficiário: | Amedea Barozzi Seabra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |