Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Autor(es)

Kitajima, Elliot Watanabe ^[1] ; Chagas, Cesar Martins ^[1] ; Braghini, Masako Toma ^[2] ; Fazuoli, Luiz Carlos ^[2] ; Locali-Fabris, Eliane Cristina ^[3] ; Salaroli, Renato Barbosa ^[1]

Afiliação do(s) autor(es)

^[1] USP, ESALQ, Depto Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

^[2] Ctr Caf Alcides Carvalho, Inst Agron, Campinas, SP - Brazil

^[3] Ctr Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scientia Agricola; v. 68, n. 4, p. 503-507, JUL-AUG 2011.
• Citações Web of Science: 13

Resumo

Coffee ringspot is a minor coffee disease caused by the nuclear type of Brevipalpus mite-transmitted virus, Coffee ringspot virus (CoRSV). Recently outbreaks of the disease in some growing regions of the state of Minas Gerais, Brazil, were registered with qualitative and quantitative yield losses. Coffea arabica was the only species registered as natural host. A survey was made on a germplasm collection of Coffea and related species kept at the Centro de Café "Alcides Carvalho", Instituto Agronômico, Campinas, state of São Paulo (SP), Brazil, to assess natural susceptibility of Coffee species, other than C. arabica and some interspecific hybrids of Coffea as well as other non-Coffea plant species to the Coffee ringspot virus (CoRSV). The following plants were found with ringspot symptoms on their leaves and/or fruits besides C. arabica L.: C. kapakata (IAC 4511), C. dewevrei cv. Excelsa, C. canephora cv. Robusta, hybrid derivative of the C. arabica × C. racemosa (IAC1195-5-6-2), C. arabica × C. dewerei (Piatã IAC 387), Híbrido de Timor CIFC 832/1 (derivative from a natural crossing between C. arabica × C. canephora) and C. racemosa. Also Psilanthus ebracteolatus, a species close to the genus Coffee was also found with ringspot lesions on their leaves. All these plants were also found infested by Brevipalpus mites identified as B. phoenicis. Infection of these plants by CoRSV was confirmed by the observation of characteristic cytopathic effects in the tissues of the lesion and by RT-PCR using a pair of primer specific for CoRSV. Only with C. racemosa RT-PCR failed to amplify the CoRSV genome. The susceptibility of P. ebracteolatus to CoRSV adds new dimension regarding its controversial taxonomic position. (AU)

• Processo FAPESP: 07/50809-0 - Mancha anular do cafeeiro: estudos sobre a variedade do vírus e sua distribuição geográfica, gama de hospedeiras, relações vírus/ácaro vetor e avaliação de fontes de resistência em Coffea
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Regular