| Texto completo | |
| Autor(es): |
Murashima, Adriana de Andrade Batista
;
Sant'Ana, Ariane M. S.
;
Faustino-Barros, Jacqueline Ferreira
;
Machado Filho, Elisio B.
;
da Silva, Lilian Costa Mendes
;
Fantucci, Marina Zilio
;
Modulo, Carolina Maria
;
Chahud, Fernando
;
Garcia, Denny Marcos
;
Rocha, Eduardo M.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 25, n. 15, p. 15-pg., 2024-08-01. |
| Resumo | |
The study evaluated the regenerative responses of the lacrimal functional unit (LFU) after lacrimal gland (LG) ablation. The LG of Wistar rats was submitted to G1) partial LG ablation, G2) partial ablation and transplantation of an allogeneic LG, or G3) total LG ablation, (n = 7-10/group). The eye wipe test, slit lamp image, tear flow, and histology were evaluated. RT-PCR analyzed inflammatory and proliferation mediators. The findings were compared to na & iuml;ve controls after 1 and 2 months (M1 and M2). G3 presented increased corneal sensitivity, and the 3 groups showed corneal neovascularization. Histology revealed changes in the LG and corneal inflammation. In the LG, there was an increase in MMP-9 mRNA of G1 and G2 at M1 and M2, in RUNX-1 at M1 and M2 in G1, in RUNX-3 mRNA at M1 in G1, and at M2 in G2. TNF-alpha mRNA rose in the corneas of G1 and G2 at M2. There was an increase in the IL-1 beta mRNA in the trigeminal ganglion of G1 at M1. Without changes in tear flow or evidence of LG regeneration, LG ablation and grafting are unreliable models for dry eye or LG repair in rats. The surgical manipulation extended inflammation to the LFU. (AU) | |
| Processo FAPESP: | 14/23211-0 - História Natural da Disfunção das Glândulas Lacrimal e Salivar na Síndrome de Sjögren |
| Beneficiário: | Eduardo Melani Rocha |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/22451-7 - Sistemas de liberação sustentada e direcionada de fármacos para o tecido epitelial |
| Beneficiário: | Renata Fonseca Vianna Lopez |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |