Employment of Magnetic Poly(Styrene-co-Triethylene Gycol Dimethacrylate) as an Immobilization Matrix for Lipase G: Application of Hexyl Oleate Synthesis and Kinetic Study

Autor(es)

de Assis, Gabrielle P. ; Silva, Vinicius S. ; da Silva, Mateus V. C. ; Rangel, Amanda B. S. ; Aguiar, Leandro G. ; de Freitas, Larissa

• Tipo de documento: Artigo Científico
• Fonte: CATALYSIS LETTERS; v. N/A, p. 11-pg., 2024-10-07.

Resumo

This study aimed to synthesize the emollient ester hexyl oleate by enzymatic catalysis using lipase G from Penicillium camemberti immobilized on the magnetic copolymer poly(styrene-co-triethylene glycol dimethacrylate) (STY-TEGDMA-M). For this, an experimental study was carried out on esterification reactions conducted in solvent-free medium, and the collected data were used to construct a mathematical model. The physical properties of the immobilized derivative were evaluated by scanning electron microscopy and Fourier-transform infrared spectroscopy. The results confirmed that lipase G was successfully immobilized onto the support, with a catalytic activity of 564.63 U g-1. The biocatalyst showed optimum performance at 45 degrees C, reaching 82% conversion after 48 h of reaction. A chromatographic method was used to confirm the formation of hexyl oleate. A thermal stability assay was conducted, showing that the biocatalyst retained 87% of its initial activity after 4 h of incubation at 60 degrees C. Mathematical modeling was based on the ping-pong bi-bi kinetic mechanism. The best model was selected according to the lowest value of the corrected Akaike information criterion (AICC = 124.776). The model revealed equilibrium constant values (Keq) ranging from 0.87 to 14.57, maximum rates of the forward reaction ranging from 0.088 to 0.090 mol L-1 min-1, and a maximum rate of the reverse reaction of 0.021 mol L-1 min-1. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 16/17833-3 - Síntese de ésteres emolientes catalisada por lipase imobilizada em copolímeros à base de estireno com propriedades magnéticas
• Beneficiário: Larissa de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/04243-7 - "Aplicação da tecnologia verde para a remediação da poluição ambiental empregando catalisadores heterogêneos à partir de suportes alternativos"
• Beneficiário: Larissa de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Regular