| Texto completo | |
| Autor(es): |
Ferreira, Rogerio S.
;
da Silva, Rodrigo A.
;
Feltran, Georgia Da S.
;
da Silva, Ericka Patricia
;
de Assis, Rahyza I. F.
;
Rovai, Emanuel Silva
;
Zambuzzi, Willian F.
;
Andia, Denise C.
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ORAL DISEASES; v. 30, n. 6, p. 11-pg., 2023-11-22. |
| Resumo | |
Background: Here, we evaluated whether the histone lysine demethylase 5B (JARID1B), is involved in osteogenic phenotype commitment of periodontal ligament cells (PDLCs), by considering their heterogeneity for osteoblast differentiation.Materials and Methods: Epigenetic, transcriptional, and protein levels of a gene set, involved in the osteogenesis, were investigated by performing genome-wide DNA (hydroxy)methylation, mRNA expression, and western blotting analysis at basal (without osteogenic induction), and at the 3rd and 10th days of osteogenic stimulus, in vitro, using PDLCs with low (l) and high (h) osteogenic potential as biological models.Results: h-PDLCs showed reduced levels of JARID1B, compared to l-PDLCs, with significant inversely proportional correlations between RUNX2 and RUNX2/p57. Epigenetically, a significant reduction in the global H3K4me3 content was observed only in h-PDLCs. Immunoblotting data reveal a significant reduction in the global H3K4me3 content, at 3 days of induction only in h-PDLCs, while an increase in the global H3K4me3 content was observed at 10 days for both PDLCs. Additionally, positive correlations were found between global H3K4me3 levels and JARID1B gene expression.Conclusions: Altogether, our results show the crucial role of JARID1B in repressing PDLCs osteogenic phenotype and this claims to pre-clinical protocols proposing JARID1B as a potential therapeutic target. (AU) | |
| Processo FAPESP: | 14/22689-3 - "Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo" |
| Beneficiário: | Willian Fernando Zambuzzi |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 16/08888-9 - "Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo" |
| Beneficiário: | Célio Junior da Costa Fernandes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 13/09650-8 - Regulação epigenética em células mesenquimais humanas |
| Beneficiário: | Denise Carleto Andia |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 18/10443-0 - Fenótipo osteogênico induzido pelo endotélio: Lições da tecnologia de iPSCs humanos e controle epigenético. |
| Beneficiário: | Rodrigo Augusto da Silva |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |