Dermatan sulfate and bone marrow mononuclear cells used as a new therapeutic strategy after arterial injury in mice

Autor(es)

Godoy, Juliana A. P. ^[1] ; Block, Daniel B. ^[1] ; Tollefsen, Douglas M. ^[2] ; Werneck, Claudio C. ^[3] ; Vicente, Cristina P. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Dept Anat Cellular Biol Physiol & Biophys, Inst Biol, BR-13083863 Sao Paulo - Brazil

^[2] Washington Univ, Sch Med, Dept Med, Div Hematol, St Louis, MO 63110 - USA

^[3] State Univ Campinas UNICAMP, Dept Biochem, Inst Biol, BR-13083863 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOTHERAPY; v. 13, n. 6, p. 695-704, JUL 2011.
• Citações Web of Science: 9

Resumo

Background aims. Previously, we have demonstrated that administration of dermatan sulfate (DS) suppresses neointima formation in the mouse carotid artery by activating heparin co-factor II. A similar suppressive effect was observed by increasing the number of progenitor cells in circulation. In this study, we investigated the combination of DS and bone marrow mononuclear cells (MNC), which includes potential endothelial progenitors, in neointima formation after arterial injury. Methods. Arterial injury was induced by mechanical dilation of the left common carotid artery. We analyzed the extension of endothelial lesion, thrombus formation, P-selectin expression and CD45(+) cell accumulation 1 and 3 days post-injury, and neointima formation 21 days post-injury. Animals were injected with MNC with or without DS during the first 48 h after injury. Results. The extension of endothelial lesion was similar in all groups 1 day after surgery; however, in injured animals treated with MNC and DS the endothelium recovery seemed to be more efficient 21 days after lesion. Treatment with DS inhibited thrombosis, decreased CD45(+) cell accumulation and P-selectin expression at the site of injury, and reduced the neointimal area by 56%. Treatment with MNC reduced the neointimal area by 54%. The combination of DS and MNC reduced neointima formation by more than 91%. In addition, DS promoted a greater accumulation of MNC at the site of injury. Conclusions. DS inhibits the initial thrombotic and inflammatory processes after arterial injury and promotes migration of MNC to the site of the lesion, where they may assist in the recovery of the injured endothelium. (AU)

• Processo FAPESP: 07/01112-6 - Efeito do dermatan sulfato na migracao, adesao e proliferacao de celulas progenitoras endoteliais: uma alternativa terapeutica para injurias arterias?
• Beneficiário: Cristina Pontes Vicente
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/11474-5 - Influência de glicosaminoglicanos na inflamação e terapia celular utilizando células progenitoras endoteliais após a lesão arterial em camundongos
• Beneficiário: Cristina Pontes Vicente
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/01119-3 - Influência do tratamento com glicosaminoglicanos no processo inflamatório e na terapia celular utilizando células progenitoras endoteliais para recuperação de lesões arteriais de camundongos.
• Beneficiário: Juliana Aparecida Preto de Godoy
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/00950-3 - Avaliação da função da fibrilina-1 na trombogênese arterial
• Beneficiário: Claudio Chrysostomo Werneck
• Modalidade de apoio: Auxílio à Pesquisa - Regular