| Texto completo | |
| Autor(es): |
Cavalcante, Taina
;
Marques, Antonio Moreira
;
Medeiros, Mariana Medina
;
Reis, Tania Carolina
;
Quina, Daniel
;
de Alencar, Bruna Cunha
;
Palmisano, Giuseppe
;
Stolf, Beatriz Simonsen
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cell Biology International; v. N/A, p. 8-pg., 2025-01-07. |
| Resumo | |
Leishmaniases affect millions of people around the world, caused by Leishmania parasites. Leishmania are transmitted by female sandflies from Phlebotominae subfamily during their blood meals. In mammals, promastigotes are phagocytosed mainly by macrophages, differentiate into amastigotes and multiply. For entry and survival in macrophages, Leishmania uses virulence factors such as surface glycoconjugates. Sialic acids (Sias) are found in terminal portions of glycoconjugates and play important roles in human pathogens. The importance of Sias was explored only in L. (L.) donovani, associated with visceral leishmaniasis in Africa, Asia and Europe. Thus, the aim of this study was to characterize Sias of Leishmania (L.) amazonensis and Leishmania (L.) infantum, related to cutaneous and visceral leishmaniasis in South America, respectively. For that, we analyzed by HPLC-FLD the Sias of promastigotes of L. (L.) amazonensis LV79 and two L. (L.) infantum strains, and of L. (L.) amazonensis axenic amastigotes and amastigotes from paw lesions of infected mice. To evaluate Sias importance in promastigotes, we treated stationary phase parasites with sialidase and infected murine and human macrophages. We detected N-Acetylneuraminic Acid in promastigotes of all strains, with greater abundance in L. (L.) infantum. We identified N-Acetylneuraminic Acid and N-Glycolylneuraminic acid in amastigotes recovered from paw lesion, but only N-Acetylneuraminic Acid in axenic amastigotes. Promastigotes treated with sialidase infected less macrophages than parasites displaying total Sias. Our results demonstrate that Sias vary between Leishmania species and between L. (L.) amazonensis life stages and plays an important role in macrophage infection by L. (L.) amazonensis and L. (L.) infantum. (AU) | |
| Processo FAPESP: | 18/18257-1 - EMU concedido no processo 14/06863-3: sistema de cromatografia líquida configurado para análise de carboidratos, aminoácidos, peptídeos e glicoproteínas |
| Beneficiário: | Giuseppe Palmisano |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 21/08915-4 - Identificação de fatores relacionados com fagocitose e sobrevivência de L. amazonensis por comparação de proteomas e análise de mutantes por CRISPR-Cas9 |
| Beneficiário: | Beatriz Simonsen Stolf |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/15549-1 - Modificações pós-traducionais nos processos biológicos e no diagnóstico da Doença de Chagas: novas abordagens metodológicas e implicações biológicas |
| Beneficiário: | Giuseppe Palmisano |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 20/04923-0 - Glicosilação do SARS-CoV-2 para identificação das características estruturais da COVID-19 |
| Beneficiário: | Giuseppe Palmisano |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |