| Texto completo | |
| Autor(es): |
Leite, Giuseppe G. F.
;
Sousa, Monica Braganca
;
Rodrigues, Larissa de Oliveira C. P.
;
Brunialti, Milena Karina Colo
;
Medina-Pestana, Jose
;
Butler, Joe M.
;
Peters-Sengers, Hessel
;
Requiao-Moura, Lucio
;
Salomao, Reinaldo
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN IMMUNOLOGY; v. 15, p. 13-pg., 2024-12-16. |
| Resumo | |
The COVID-19 pandemic has significantly impacted global health, especially in vulnerable populations like kidney transplant recipients (KTRs). Recently, mass spectrometry-based proteomics has emerged as a powerful tool to shed light on a broad spectrum of dysregulated biological processes in KTRs with COVID-19. In this study, we prospectively collected blood samples from 17 COVID-19-positive KTRs and 10 non-infected KTRs between May and September 2020. Using tandem mass tag-based quantitative proteomics, we analyzed peripheral blood mononuclear cells (PBMCs), plasma protein biomarkers, and lymphocyte counts, followed by bioinformatics analysis. Our results revealed significant proteomic alterations in COVID-19-infected KTRs, particularly in pathways related to glycolysis, glucose metabolism, and neutrophil degranulation. Additionally, we observed an altered immune response characterized by elevated cytokines and decreased lymphocyte counts. Notably, KTRs with AKI exhibited worse clinical outcomes, including higher rates of ICU admission and mechanical ventilation. Comparative analysis of PBMC proteomic profiles between AKI and non-AKI patients identified distinct immune-related pathways, with AKI patients showing marked changes in innate immune responses, particularly neutrophil degranulation. Furthermore, we observed a negative correlation between T cell counts and neutrophil degranulation, suggesting a role for immune dysregulation in COVID-19. Our findings provide critical insights into the immune and metabolic responses in COVID-19-infected KTRs, especially those with AKI, highlighting the need for focused research and therapeutic strategies targeting immune dysregulation in this high-risk population. (AU) | |
| Processo FAPESP: | 17/21052-0 - Sepse: mecanismos, alvos terapêuticos e epidemiologia |
| Beneficiário: | Reinaldo Salomão |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/05110-2 - Estudo de coorte prospectiva para avaliação de aspectos clínicos, virológicos e de resposta do hospedeiro em pacientes com COVID-19 |
| Beneficiário: | Reinaldo Salomão |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/20532-3 - Análise proteômica quantitativa nas amostras de células mononucleares (PBMC) e polimorfonucleares (PMN) de pacientes com Sepse e voluntários sadios, com foco em proteínas relacionadas ao metabolismo energético e oxidativo, além da função mitocondrial |
| Beneficiário: | Giuseppe Gianini Figueiredo Leite |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |