Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes

Autor(es)

Francisco, C. L. ^[1] ; Jorge, A. M. ^[1] ; Dal-Pai-Silva, M. ^[2] ; Carani, F. R. ^[2] ; Cabeco, L. C. ^[2] ; Silva, S. R. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Dept Anim Prod, BR-18618000 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencia, Dept Morfol, BR-18618000 Botucatu, SP - Brazil

^[3] Univ Tras Os Montes & Alto Douro, CECAV, Dept Anim Sci, P-5000801 Vila Real - Portugal

• Tipo de documento: Artigo Científico
• Fonte: MEAT SCIENCE; v. 88, n. 3, p. 535-541, JUL 2011.
• Citações Web of Science: 5

Resumo

This study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC lib isoforms was not found. Only three isoforms of MyHC (Ila, Ilx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/53487-3 - Caracterizacao histologica e bioquimica dos musculos longissimus dorsi e semitendinosus de bubalinos mediterraneos nao castrados abatidos em diferentes pesos.
• Beneficiário: Caroline de Lima Francisco
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/59965-4 - Características qualitativas, histoquímicas e composição de proteínas miofibrilares dos músculos Longissimus dorsi e Semitendinosus de bubalinos mediterrâneos nao-cadastrados abatidos em diferentes pesos
• Beneficiário: Andre Mendes Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular