Immunogenic recombinant Mayaro virus-like particles present natively assembled glycoprotein

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Kim, Young Chan ; Watanabe, Yasunori ; Arlen-Celina, Luecke ; Song, Xiyong ; Souza, Raquel de Oliveira ; Stass, Robert ; Azar, Sasha R. ; Rossi, Shannan L. ; Claser, Carla ; Kuemmerer, Beate Mareike ; Crispin, Max ; Bowden, Thomas A. ; Huiskonen, Juha T. ; Reyes-Sandoval, Arturo

• Tipo de documento: Artigo Científico
• Fonte: NPJ VACCINES; v. 9, n. 1, p. 13-pg., 2024-12-17.

Resumo

Virus-like particles (VLPs) are an established vaccine platform and can be strong immunogens capable of eliciting both humoral and cellular immune responses against a range of pathogens. Here, we show by cryo-electron microscopy that VLPs of Mayaro virus, which contain envelope glycoproteins E1-E2 and capsid, exhibit an architecture that closely resembles native virus. In contrast to monomeric and soluble envelope 2 (E2) glycoprotein, both VLPs as well as the adenovirus and modified vaccinia virus Ankara (MVA) vaccine platforms expressing the equivalent envelope glycoproteins E1-E2, and capsid induced highly neutralising antibodies after immunisation. The levels of neutralising antibodies elicited by the viral-vectored vaccines of structural proteins and VLPs increased significantly upon boosting. Immunisation of Mayaro virus VLPs in mice with or without an adjuvant (poly:IC) yielded similar levels of neutralising antibodies suggesting that the VLPs may be used for immunisation without the need for an adjuvant. A single or two doses of non-adjuvanted 5 mu g of MAYV VLP vaccination provided significant protection against viremia and MAYV-induced foot swelling in the C57BL/6 mouse challenge model. MAYV VLPs represent a non-infectious vaccine candidate, which may constitute a complementary option for future immunisation strategies against this important emerging alphavirus. (AU)

• Processo FAPESP: 22/15124-6 - Estudo da modulação da resposta imune humoral e celular induzida pelos vírus Mayaro e Chikungunya em camundongos coinfectados
• Beneficiário: Raquel de Oliveira Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/24470-0 - Estudo das alterações imunopatológicas induzidas durante a coinfecção com Alphavirus e Malária em modelo murino
• Beneficiário: Carla Claser
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores