| Texto completo | |
| Autor(es): Mostrar menos - |
Romano, Beatriz Coimbra
;
de Araujo, Iana Mizumukai
;
Ribeiro, Mariana S. P.
;
Silva, Luciana T. Parreiras e
;
Dick-de-Paula, Ingid
;
Fukada, Sandra Y.
;
Porto, Felipe Manoel
;
Jorgetti, Vanda
;
Pereira, Francisco de Assis
;
Elias, Lucila Leico Kagohara
;
de Paula, Francisco Jose Albuquerque
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JBMR PLUS; v. 9, n. 1, p. 9-pg., 2024-12-13. |
| Resumo | |
The present study was designed to evaluate the influence of a high-protein diet under conditions of calorie restriction (CR) in the muscle, adipose tissue, bone, and marrow adipose tissue (MAT). It included three groups of 20 female Wistar Hannover rats, fed with the following diets for 8 wk: control group (C) fed with an AIN93M diet, CR group (R) fed with an AIN-93M diet modified to 30% CR, and CR + high-protein group (H) fed with an AIN-93M diet modified to 30% CR with 40% protein. Body composition was determined by DXA. The femur was used for histomorphometry and the estimation of adipocytes. Microcomputed tomography (mu CT) was employed to analyze the bone structure. Hematopoietic stem cells from the bone marrow were harvested for osteoclastogenesis. Body composition revealed that the gain in lean mass surpassed the increase in fat mass only in the H group. Bone histomorphometry and mu CT showed that a high-protein diet did not mitigate CR-induced bone deterioration. In addition, the number of bone marrow adipocytes and the differentiation of hematopoietic stem cells into osteoclasts were higher in H than in the other groups. These results indicated that under CR, a high-protein diet was beneficial for muscle mass. However, as the mu CT scanning detected significant bone deterioration, this combined diet might accentuate the detrimental effect on the skeleton caused by CR. Remarkably, the H group rats exhibited greater MAT expansion and elevated hematopoietic stem cell differentiation into osteoclasts than the CR and control counterparts. These data suggest that a high protein may not be an appropriate strategy to preserve bone health under CR conditions. A high-protein diet is widely used to enhance muscle mass gain. The relationship between a high-protein diet and lean mass is well established, but its influence on bone is yet to be determined. This study was designed to compare three groups of female Wistar Hannover rats: control (C) fed with a regular chow diet, calorie restriction (CR) fed with 30% fewer calories in comparison to C, and CR with 30% fewer calories than C along with 40% more protein (H). The results showed that a high-protein diet benefits muscle mass, even under CR. However, a low-calorie and high-protein diet accentuates the detrimental impact that CR has on bone. (AU) | |
| Processo FAPESP: | 21/03152-2 - Interação entre tecido adiposo, muscular e ósseo na anorexia nervosa |
| Beneficiário: | Iana Mizumukai de Araujo |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/18071-5 - Homeostase hidroeletrolítica e energética: do metabolismo celular aos sistemas endócrinos em diferentes fases do desenvolvimento |
| Beneficiário: | Lucila Leico Kagohara Elias |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |