| Texto completo | |
| Autor(es): Mostrar menos - |
Thome, Vanessa
;
Ferreira, Pedro B.
;
Lubini, Greice
;
Nogueira, Fernanda M.
;
Strini, Edward J.
;
Pinoti, Vitor F.
;
Cruz, Joelma O.
;
San Martin, Juca A. B.
;
Quiapim, Andrea C.
;
Dasilva, Luis L. P.
;
Goldman, Maria Helena S.
Número total de Autores: 11
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| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 26, n. 1, p. 23-pg., 2025-01-01. |
| Resumo | |
In the Nicotiana tabacum flower development study, we identified SCI1 (Stigma/style Cell-cycle Inhibitor 1), a regulator of cell proliferation. SCI1 interacts with NtCDKG;2 (N. tabacum Cyclin-Dependent Kinase G;2), a homolog of human CDK11, which is responsible for RanGTP-dependent microtubule stabilization, regulating spindle assembly rate. In a Y2H screening of a cDNA library using NtCDKG;2 as bait, a RanBP1 (Ran-Binding Protein 1) was revealed as its interaction partner. RanBP1 is an essential regulatory protein of the RanGTPase system, contributing to the formation of the Ran gradient, which modulates different important cellular processes. RanBP1 is crucial in the nuclear import/export machinery during interphase and spindle checkpoint formation during cell division. These processes are well studied in animals, but very little is known about them in plants. We confirmed NtCDKG;2 and NtRanBP1 interaction by pairwise Y2H and characterized the localization of both proteins during plant cell division. We demonstrated the presence of NtRanBP1 in the cytoplasm during interphase and its nuclear arrest at mitosis onset. Meanwhile, we showed that NtCDKG;2 is localized in the mitotic spindle during cell division, indicating an analogous function to the human CDK11. We propose that the phosphorylation of the nuclear export signal at RanBP1 by NtCDKG;2 may be responsible for the reported nuclear arrest. (AU) | |
| Processo FAPESP: | 12/50562-2 - Estudo de SCI1 e suas interações proteína-proteína, que resultam na inibição do ciclo celular |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/24774-1 - A importância do gene SCI1 no meristema floral, sua regulação transcricional e conservação evolutiva |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/20486-3 - Decifrando SCI1, sua expressão durante o desenvolvimento floral e os mecanismos de ação para o controle da proliferação celular |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 23/12687-2 - Estudo do desenvolvimento floral e da cinética do crescimento radicular em plantas transgênicas com níveis alterados de expressão de NtRH35 (NtDDX35) |
| Beneficiário: | Fernanda Mayara Nogueira |
| Modalidade de apoio: | Bolsas no Brasil - Programa Fixação de Jovens Doutores |