Absence of inhibitory effects of two new glucanases on streptococcus mutans growth

Autor(es)

Mateus Xavier-Queiroz ; Pedro Ricardo Vieira Hamann ^[2] ; Heidi Aarø Åmdal ^[3] ; Igor Polikarpov ^[4] ; Fernanda Cristina Petersen ^[5] ; Antônio Pedro Ricomini-Filho ^[6]

• Tipo de documento: Artigo Científico
• Fonte: Braz. J. Oral Sci.; v. 23, 2025-01-10.

Resumo

Glucanohydrolases have shown promise in degrading exopolysaccharides in cariogenic biofilms, making them a potential strategy for biofilm control without disrupting the oral microbiota. However, their direct antimicrobial effects remain unclear. Aim To determine the antimicrobial activity on S. mutans of two newly discovered glucanases characterized by our group, PmGH87 (mutanase) from Prevotella melaninogenica and CoGH66 (dextranase) from Capnocytophaga ochracea, using a commercial dextranase from Penicillum sp. as a control. Methods Their effects on growth were assessed using a luciferase reporter system coupled with the promoter of the ldh gene in Streptococcus mutans. Results Quantification of optical density and luminescence over a 10-hour growth period revealed that the commercial dextranase exhibited inhibitory effects on S. mutans growth. However, these effects were neutralized by heat treatment, suggesting the presence of a heat-sensitive contaminant or an additional antimicrobial property associated with the commercial dextranase from Penicillum sp. On the other hand, the purified mutanase and dextranase enzymes had no inhibitory effect on S. mutans growth. Conclusion In conclusion, the absence of inhibitory effects on S. mutans growth by the newly discovered enzymes emphasizes their potential for biofilm control while preserving the delicate balance of the oral microbiota and preventing the emergence of resistance. (AU)

• Processo FAPESP: 21/08780-1 - Degradação enzimática de exopolissacharídeos de biofilmes microbianos: abordagens de biofísica estrutural, biotecnologia molecular e bioquímica sintética em estudos de CAZymes em busca de novas ferramentas enzimáticas para tratamentos antimicrobianos
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/15092-1 - Bioatividade de enzimas na degradação da matriz do biofilme para controle de doenças orais
• Beneficiário: Antônio Pedro Ricomini Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/07897-8 - Estudos bioquímicos e estruturais de CAZymes com potencial na degradação de exopolissacharídeos de biofilmes microbianos
• Beneficiário: Pedro Ricardo Vieira Hamann
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado