| Texto completo | |
| Autor(es): |
Florezi, Giovanna P.
;
Labate, Monica Teresa Veneziano
;
Labate, Carlos Alberto
;
Barone, Felippe P.
;
Lourenco, Silvia Vanessa
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ORAL DISEASES; v. N/A, p. 13-pg., 2025-03-27. |
| Resumo | |
Objectives: Primary Sj & ouml;gren's disease (SjD) is a slow-progressing autoimmune disease that affects salivary and lacrimal glands, causing dry eyes (xerophthalmia) and dry mouth (xerostomia). Diagnosing SjD involves clinical, serological, and histological assessments, but reliable biomarkers are lacking. Methods: This study analyzed the metabolic and proteomic profiles of 19 female SjD patients (based on ACR-EULAR criteria) compared to 20 healthy individuals. Saliva and blood samples were analyzed using mass spectrometry and chromatography. Proteomic analysis was performed with the nanoElute nanoflow system coupled to a timsTof-Pro mass spectrometer, and metabolic profiling with a GC-TOF/MS Pegasus HT. Results: Statistical tests identified significant differences in metabolites and proteins between SjD patients and controls. Metabolome analysis revealed changes in amino acid synthesis, purine and lipid metabolism, and exposure to external compounds. Proteomic analysis indicated immune-related proteins and inflammatory lipid metabolism. GNAI2, B2MG, NGAL, SLUR2, HS90, SODC, and A2GL emerged as potential biomarkers for SjD. Conclusion: This study demonstrates the potential of high-performance techniques in identifying biomarkers for SjD diagnosis and prognosis. Data are available via ProteomeXchange (PXD055629). (AU) | |
| Processo FAPESP: | 17/11806-7 - Sialadenite na síndrome de Sjögren e no lúpus eritematoso: correlação clínico-patológica e análises proteômica e metabolômica da saliva |
| Beneficiário: | Silvia Vanessa Lourenço |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/00419-5 - Genômica funcional: engenharia reversa por meio de métodos de alta performance na saliva e sangue de pacientes de Síndrome de Sjögren |
| Beneficiário: | Giovanna Piacenza Florezi |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 22/12807-5 - Xerostomia na pré e pós menopausa e sua interface com a saúde bucal da mulher: investigação da bancada ao leito |
| Beneficiário: | Silvia Vanessa Lourenço |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/12702-6 - Sialadenite lúpica: análise dos metabólitos salivares e sua possível relação com aspectos peculiares da morfologia glandular |
| Beneficiário: | Felippe Pereira Barone |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |