Advancing Nanotechnology: Targeting Biofilm-Forming Bacteria with Antimicrobial Peptides

Autor(es)

Campos, Julia Valladares ; de Pontes, Janaina Teixeira Costa ; Canales, Christian Shleider Carnero ; Roque-Borda, Cesar Augusto ; Pavan, Fernando Rogerio

• Tipo de documento: Artigo Científico
• Fonte: BME FRONTIERS; v. 6, p. 22-pg., 2025-03-04.

Resumo

Nanotechnology offers innovative solutions for addressing the challenges posed by biofilm-forming bacteria, which are highly resistant to conventional antimicrobial therapies. This review explores the integration of pharmaceutical nanotechnology with antimicrobial peptides (AMPs) to enhance the treatment of biofilm-related infections. The use of various nanoparticle systems-including inorganic/metallic, polymeric, lipid-based, and dendrimer nanostructures-provides promising avenues for improving drug delivery, targeting, and biofilm disruption. These nanocarriers facilitate the penetration of biofilms, down-regulate biofilm-associated genes, such as ALS1, ALS3, EFG1, and HWP1, and inhibit bacterial defense mechanisms through membrane disruption, reactive oxygen species generation, and intracellular targeting. Furthermore, nanoparticle formulations such as NZ2114-NPs demonstrate enhanced efficacy by reducing biofilm bacterial counts by several orders of magnitude. This review highlights the potential of combining nanotechnology with AMPs to create novel, targeted therapeutic approaches for combatting biofilm-related infections and overcoming the limitations of traditional antimicrobial treatments. (AU)

• Processo FAPESP: 23/15838-1 - Avaliação do efeito in vitro dos análogos CR22-DKs12, CR22-BR04 e CR21-BR11 do peptídeo antimicrobiano B1CTcu5 na inibição de bombas de efluxo frente a infecção de macrófagos por Mycobacterium tuberculosis.
• Beneficiário: Julia Valladares Campos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/16573-3 - Estudos in vitro e in vivo de análogos do peptídeo antimicrobiano B1CTcu5 encapsulados em micropartículas colón-específicas frente ao Mycobacterium tuberculosis
• Beneficiário: Cesar Augusto Roque Borda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 23/01664-1 - Síntese e caracterização de análogos do peptídeo antimicrobiano "B1CTcu5" encapsulados em micropartículas colón-específicas e estudos in vitro e in vivo frente ao Mycobacterium tuberculosis
• Beneficiário: Fernando Rogério Pavan
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/14603-5 - Descoberta e desenho de fármacos: análogos do peptídeo antimicrobiano B1CTcu5 promissores contra o Mycobacterium tuberculosis
• Beneficiário: Cesar Augusto Roque Borda
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado