Rat skin wound healing induced by alternagin-C, a disintegrin-like, Cys-rich protein from Bothrops alternatus venom

Autor(es)

Correia Sant'Ana, Estela Maria ^[1] ; Cacao Paiva Gouvea, Cibele Marli ^[2] ; Quagliotti Durigan, Joao Luiz ^[1] ; Cominetti, Marcia Regina ^[1] ; Pimentel, Edson Rosa ^[3] ; Selistre-de-Araujo, Heloisa Sobreiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Ciencias Fisiol, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Alfenas, Inst Ciencias Nat, Alfenas, MG - Brazil

^[3] Univ Fed Sao Carlos, Dept Anat Biol Celular & Fisiol & Biofis, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL WOUND JOURNAL; v. 8, n. 3, p. 245-252, JUN 2011.
• Citações Web of Science: 13

Resumo

Alternagin-C (ALT-C) is a disintegrin-like, Cys-rich protein isolated from Bothrops alternatus snake venom, which has been shown to induce in vivo angiogenesis. Therefore, this protein could be interesting as a new approach for tissue regeneration studies. Here the effects of ALT-C on fibroblasts and inflammatory cells, collagen type III and type I and TGF-alpha expression in a rat wounded skin model were studied. Thirty-five male Wistar rats (weight 270 +/- 20 g) were divided into seven groups with five animals in each of the following groups: a control group which wounded animals received treatment with natrozol (R) gel only; ALT-C10, ALT-C60 and ALT-C100 groups of wounded animals that were treated with the same amount of gel containing 10, 60 and 100 ng of ALT-C, respectively. Animals were treated once a day with 20 mu l of gel associated or not with ALT-C for 1, 3, 5 or 7 days. ALT-C treatment increased the fibroblast density, collagen deposition and accelerated the inflammatory process, mostly in the ALT-C60 group. These results indicate that ALT-C improves wound repair process in rat skin. Thus, ALT-C could be a candidate to the development of a novel therapeutic strategy for wounded skin repair. (AU)

• Processo FAPESP: 08/09408-4 - Efeito da eletroestimulação sobre a atrofia no músculo quadríceps femoral após ruptura do ligamento cruzado anterior em ratos
• Beneficiário: João Luiz Quagliotti Durigan
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado