Fluoride effect on the secretory-stage enamel organic extracellular matrix of mice

Autor(es)

Mofatto, Luciana Souto ^[1] ; Frozoni, Marcos R. S. ^[1] ; do Espirito Santo, Alexandre R. ^[2] ; Guimaraes, Gustavo Narvaes ^[1] ; de Souza, Ana Paula ^[1] ; Vidal, Benedicto de Campos ^[3] ; Peres Line, Sergio Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Morphol, Piracicaba, SP - Brazil

^[2] Fed Univ Bahia UFBA, Inst Hlth Sci, Dept Biomorphol, Salvador, BA - Brazil

^[3] Univ Campinas UNICAMP, Inst Biol, Dept Cell Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Connective Tissue Research; v. 52, n. 3, p. 212-217, JUN 2011.
• Citações Web of Science: 1

Resumo

The formation of an ordered enamel organic extracellular matrix (EOECM) seems to be a crucial step for the proper formation of the enamel mineral phase. The ordered supramolecular structure of the EOECM in the secretory stage can be analyzed using polarizing microscopy, as it is strongly birefringent. Excessive fluoride (F) ingestion during tooth development can cause enamel fluorosis, leading to increased porosity in mature enamel. We analyzed the effects of F on the birefringence of the EOECM in the A/J, CBA, and DBA/2 strains of mice given 0, 11.25, and 45 ppm of fluoride in drinking water. In the CBA and DBA/2 strains, the 11.25 and 45 ppmF groups presented a significant decrease in optical retardation (OR) when compared with the respective 0 (CBA 11.25 ppmF p = 0.0056 and 45 ppmF p < 0.0001; DBA/2 11.25 and 45 ppmF p < 0.05). ORs in A/J 0 ppmF were significantly higher than in 45 (p < 0.0001). The enamel of the A/J strain was more severely affected by fluoride than it was in the other strains of mice and exhibited the lowest levels of fluoride in plasma, whereas its normal secretory enamel presented a significantly higher protein absorbance than it did in CBA and DBA mice (p = 0.0099 and p = 0.0025, respectively). The results showed that experimental fluorosis can alter the supramolecular organization of EOECM in the secretory stage of amelogenesis and that the susceptibility to dental fluorosis seems to be influenced by the inherent characteristics of the developing enamel. (AU)

• Processo FAPESP: 04/10994-4 - Defeitos na formação do órgão dental
• Beneficiário: Sergio Roberto Peres Line
• Modalidade de apoio: Auxílio à Pesquisa - Temático