Amniotic Fluid Interleukin-1 Beta and Interleukin-6, but not Interleukin-8 Correlate with Microbial Invasion of the Amniotic Cavity in Preterm Labor

Autor(es)

Marconi, Camila ^[1] ; de Andrade Ramos, Bruna Ribeiro ^[1] ; Peracoli, Jose Carlos ^[2] ; Donders, Gilbert G. G. ^{[3, 4]} ; da Silva, Marcia Guimaraes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Gynecol & Obstet, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

^[3] Femicare Clin Res Women, Tienen - Belgium

^[4] Univ Hosp Gasthuisberg, B-3000 Leuven - Belgium

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Reproductive Immunology; v. 65, n. 6, p. 549-556, JUN 2011.
• Citações Web of Science: 57

Resumo

Problem We compared the frequency of intra-amniotic infection in preterm labor (PL) with women not in labor, and correlated infection with amniotic fluid (AF) cytokines. Detailed identification of species, especially mycoplasmata, was tried to improve our understanding of the pathogenesis of PL. Method of study AF from 20 women with PL and 20 controls were evaluated. Infection was detected by PCR for Mycoplasma hominis, Ureaplasma urealyticum and 16S rRNA bacterial gene, which was cloned and sequenced for bacterial identification. Interleukin (IL)-1 beta, IL-6, IL-8 and tumor necrosis factor (TNF)-alpha levels were measured by ELISA. Results Frequency of intra-amniotic infection is higher in PL (40.0%). Sequencing-based method identified Bacteroides fragilis, Prevotella bivia and Leptotrichia amnionii, in addition to Mycoplasma species detected by PCR. AF infection correlated with increased IL-1 beta and IL-6 levels. Conclusion The frequency of intra-amniotic infection, especially M. hominis, in PL women who delivered with 7 days, is high and correlates with high IL-1 beta and IL-6 levels, but not IL-8. (AU)

• Processo FAPESP: 07/50726-7 - Correlação entre presença de Mycoplasma hominis e Ureaplasma urealyticum e níveis de IL-6 e IL-10 no líquido aminiótico de gestantes em trabalho de parto prematuro e corioamnionite
• Beneficiário: Bruna Ribeiro de Andrade Ramos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 07/52758-3 - Correlação entre presença de Mycoplasma hominis e Ureaplasma urealyticum e níveis de citocinas no líquido amniótico de gestantes com trabalho de parto prematuro
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular