The association of DNase I with antimicrobial photodynamic therapy affects Candida albicans gene expression and promotes immunomodulatory effects in mice with candidiasis

Autor(es)

Jordao, Claudia Carolina ; Klein, Marlise Inez ; Barbugli, Paula Aboud ; Ferrisse, Tulio Morandin ; de Moraes, Juliana Cerini Grassi ; Pavarina, Ana Claudia

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 24, n. 5, p. 12-pg., 2025-05-05.

Resumo

This study evaluated Candida albicans gene expression and local inflammatory response from mice with oral candidiasis that were treated with DNase I plus antimicrobial photodynamic therapy (aPDT). DNase I was applied, followed by aPDT mediated by Photodithazine (R) and LED light, singly or combined for five consecutive days. A group was only inoculated with fluconazole-susceptible (CaS) or-resistant (CaR) C. albicans strains. Immediately and 7 days after treatments, C. albicans colonies recovered from mice tongues were used for gene expression evaluation. Tongues from mice killed 24 h and 7 days after treatments were used to evaluate the host's inflammatory cytokines using cytometric bead array. A reduction occurred in the gene expression related to adhesion to the host substrate, the biofilm extracellular matrix, and oxidative stress of CaS and CaR recovered from mice treated with DNAse I plus aPDT. Increased production of IL-6, TNF-alpha, and MCP-1 occurred in mice infected with CaS but not as much for those infected with CaR in the groups treated with DNase I plus aPDT and aPDT. DNase I plus aPDT decreased the expression of the fungal genes evaluated and increased local inflammatory response, which helped to fight the infection. (AU)

• Processo FAPESP: 20/09332-0 - Avaliação do efeito da terapia fotodinâmica antimicrobiana associada a DNAse no tratamento de candidose oral em camundongos infectados com Candida albicans
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/01191-0 - Indução de tolerância e caracterização do biofime de Candida albicans submetido à terapia fotodinâmica antimicrobiana
• Beneficiário: Túlio Morandin Ferrisse
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/27634-6 - Avaliação do efeito da terapia fotodinâmica antimicrobiana associada a DNAse no tratamento de Candidose Oral em camundongos infectados com Candida albicans
• Beneficiário: Cláudia Carolina Jordão
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs