| Texto completo | |
| Autor(es): |
Jordao, Claudia Carolina
;
Klein, Marlise Inez
;
Barbugli, Paula Aboud
;
Ferrisse, Tulio Morandin
;
de Moraes, Juliana Cerini Grassi
;
Pavarina, Ana Claudia
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 24, n. 5, p. 12-pg., 2025-05-05. |
| Resumo | |
This study evaluated Candida albicans gene expression and local inflammatory response from mice with oral candidiasis that were treated with DNase I plus antimicrobial photodynamic therapy (aPDT). DNase I was applied, followed by aPDT mediated by Photodithazine (R) and LED light, singly or combined for five consecutive days. A group was only inoculated with fluconazole-susceptible (CaS) or-resistant (CaR) C. albicans strains. Immediately and 7 days after treatments, C. albicans colonies recovered from mice tongues were used for gene expression evaluation. Tongues from mice killed 24 h and 7 days after treatments were used to evaluate the host's inflammatory cytokines using cytometric bead array. A reduction occurred in the gene expression related to adhesion to the host substrate, the biofilm extracellular matrix, and oxidative stress of CaS and CaR recovered from mice treated with DNAse I plus aPDT. Increased production of IL-6, TNF-alpha, and MCP-1 occurred in mice infected with CaS but not as much for those infected with CaR in the groups treated with DNase I plus aPDT and aPDT. DNase I plus aPDT decreased the expression of the fungal genes evaluated and increased local inflammatory response, which helped to fight the infection. (AU) | |
| Processo FAPESP: | 20/09332-0 - Avaliação do efeito da terapia fotodinâmica antimicrobiana associada a DNAse no tratamento de candidose oral em camundongos infectados com Candida albicans |
| Beneficiário: | Ana Cláudia Pavarina |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/01191-0 - Indução de tolerância e caracterização do biofime de Candida albicans submetido à terapia fotodinâmica antimicrobiana |
| Beneficiário: | Túlio Morandin Ferrisse |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/27634-6 - Avaliação do efeito da terapia fotodinâmica antimicrobiana associada a DNAse no tratamento de Candidose Oral em camundongos infectados com Candida albicans |
| Beneficiário: | Cláudia Carolina Jordão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |