Impact of egg incubation on hemocyte recruitment and susceptibility of Galleria mellonella larvae to pathogens

Autor(es)

Carmo, Paulo Henrique Fonseca ; de Lima, Patricia Michelle Nagai ; Silva, Fabiana Alves ; Ribeiro, Jaqueline Lemes ; Junqueira, Juliana Campos ; Garcia, Maira Terra

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 16, p. 12-pg., 2025-06-03.

Resumo

Introduction Galleria mellonella is widely used as an in vivo model in microbiological and toxicological studies due to its reproducibility, low cost, and ease of handling. However, maintaining homogeneous laboratory colonies presents challenges. This study evaluated whether different incubation methods influence the larval immune response against Staphylococcus aureus, Candida albicans, and Escherichia coli.Methods Four experimental groups were established: (1) Traditional group (TG), eggs stored at 27 degrees C; (2) Immediate group (IG), eggs stored at 16 degrees C for 120 days before incubation at 27 degrees C; (3) Gradual group (GG), temperature gradually reduced from 27 degrees C to 16 degrees C over 98 days, then increased back; and (4) Frozen group (FG), eggs stored at freezing temperatures.Results and discussion FG eggs were non-viable, and IG and GG showed delayed development. IG larvae were more susceptible to infections, while GG larvae resembled TG. Thus, GG can serve as an effective alternative rearing method. (AU)

• Processo FAPESP: 24/04696-4 - Identificação de substâncias com atividade antifúngica a partir da hemolinfa e nódulos de melanização de Galleria mellonella imunizadas com Candida albicans
• Beneficiário: Maíra Terra Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular