Host genetic background affects regulatory T-cell activity that influences the magnitude of cellular immune response against Mycobacterium tuberculosis

Autor(es)

Paula, Marina Oliveira ; Fonseca, Denise Morais ; Wowk, Pryscilla Fanini ; Gembre, Ana Flavia ; Fedatto, Paola Fernanda ; Sergio, Ssia Alves ; Silva, Celio Lopes ; Deperon Bonato, Vania Luiza ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Sch Med Ribeirao Preto, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunology and Cell Biology; v. 89, n. 4, p. 526-534, MAY-JUN 2011.
• Citações Web of Science: 15

Resumo

Using two mouse strains with different abilities to generate interferon (IFN)-gamma production after Mycobacterium tuberculosis infection, we tested the hypothesis that the frequency and activity of regulatory T (Treg) cells are influenced by genetic background. Our results demonstrated that the suppressive activity of spleen Treg cells from infected or uninfected BALB/c mice was enhanced, inhibiting IFN-gamma and interleukin (IL)-2 production. Infected C57BL/6 mice exhibited a decrease in the frequency of lung Treg cells and an increased ratio CD4(+):CD4(+)Foxp3(+) cells compared with infected BALB/c mice and uninfected C57BL/6 mice. Moreover, infected C57BL/6 mice also had a decrease in the immunosuppressive capacity of spleen Treg cells, higher lung IFN-gamma and IL-17 production, and restricted the infection better than BALB/c mice. Adoptive transfer of BALB/c Treg cells into BALB/c mice induced an increase in bacterial colony-forming unit (CFU) counts. Furthermore, BALB/c mice treated with anti-CD25 antibody exhibited lung CFU counts significantly lower than mice treated with irrelevant antibody. Our results show that in BALB/c mice, the Treg cells have a stronger influence than that in C57BL/6 mice. These data suggest that BALB/c and C57BL/6 mice may use some different mechanisms to control M. tuberculosis infection. Therefore, the role of Treg cells should be explored during the development of immune modulators, both from the perspective of the pathogen and the host. Immunology and Cell Biology (2011) 89, 526-534; doi:10.1038/icb.2010.116; published online 19 October 2010 (AU)

• Processo FAPESP: 04/15204-1 - Avaliacao do papel das celulas t regulatorias cd4+cd25+ em camundongos infectados com mycobacterium tuberculosis
• Beneficiário: Marina Oliveira e Paula
• Modalidade de apoio: Bolsas no Brasil - Doutorado