| Texto completo | |
| Autor(es): Mostrar menos - |
Pereira, Edmundo L. R.
;
Maranhao, Raul C.
;
Dias, Michelle N. C.
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dos Santos, Ijair R.
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dos Santos, Carolina Ramos
;
Hamoy, Moises
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Feio, Danielle Cristine A.
;
Carvalho, Priscila O.
;
Bazioli, Jaqueline M.
;
Morikawa, Aleksandra T.
;
Gomes-Leal, Walace
Número total de Autores: 11
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| Tipo de documento: | Artigo Científico |
| Fonte: | Clinics; v. 80, p. 9-pg., 2025-01-01. |
| Resumo | |
Background This study aimed to investigate the effects of LDE-MTX on acute cerebral infarction. The study focuses on how LDE-MTX can influence the outcomes of ischemic stroke in a rat model, specifically examining its neuroprotective properties. Methods Radioactively labeled LDE uptake by brain tissue was determined after IV injection in rats with Endothelin-1 (ET-1)-induced cortical ischemic stroke (n = 11) and controls (n = 18). Two groups of 5 animals were treated with LDE-MTX (1 mg/kg, IV) or LDE-alone 4 h post-stroke induction. After 7days, tissues were analyzed by immunohistochemistry for neuronal bodies, astrocytes, and microglia. Results LDE uptake was fivefold higher in ischemic rats than in controls (p = 0.0003). LDE-MTX significantly inhibited microglial activation, resulting in a tenfold decrease in activated macrophages, and increased neuronal survival by 319 % in the periinfarct area. LDE-MTX had no effect on astrocytosis or primary infarct size. Conclusions LDE-MTX demonstrated neuroprotective effects and shows potential as a novel strategy to limit ischemic stroke damage. The results suggest that LDE-MTX could be a promising treatment option for reducing ischemic damage in stroke patients, particularly through its effect on microglial activation and neuronal survival. (AU) | |
| Processo FAPESP: | 20/16215-0 - Nanopartículas lipídicas (LDE) como veículos de quimioterápicos: novo paradigma para a terapêutica médica |
| Beneficiário: | Raul Cavalcante Maranhao |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |