| Autor(es): |
de Oliveira, Rafael Ramos
[1]
;
Novaes, Jr., Arthur Belem
[2, 1]
;
Garlet, Gustavo P.
[3]
;
de Souza, Raphael F.
[4]
;
Taba, Jr., Mario
[1]
;
Sato, Sandra
[4]
;
de Souza, Sergio L. S.
[1]
;
Palioto, Daniela B.
[1]
;
Grisi, Marcio F. M.
[1]
;
Feres, Magda
[5]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Bucco Maxillo Facial Surg & Traumatol & Peri, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, BR-14040904 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Dept Dent Mat & Prostheses, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[5] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos, SP - Brazil
Número total de Afiliações: 5
|
The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP), or with the association of treatments (SRP + aPDT). Plaque samples were collected at baseline, 1, 3, and 4 weeks, and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-alpha), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16 S rRNA gene were evaluated through real-time PCR. The results shows that the levels of the majority of the species were reduced 1 week post-therapy for all treatments, however, an increase in counts of Prevotella intermedia (p = 0.00), Prevotella. nigrescens (p = 0.00) and Tannerella forsythia (p = 0.00) was observed for aPDT and SRP + aPDT. After 4 weeks, a regrowth of Porphyromonas gingivalis (p = 0.00) and Treponema denticola (p = 0.00), was observed for all treatments. Also, a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (p = 0.00). For the cytokine pattern, the results were similar for all treatments, and a reduction in the expression of cytokines and bacterial load was observed throughout the study. Our results suggest that SRP, aPDT in a single application, and SRP + aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis. (AU) |