The effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. Microbiological profile and cytokine pattern in the dog mandible

Autor(es)

de Oliveira, Rafael Ramos ^[1] ; Novaes, Jr., Arthur Belem ^{[2, 1]} ; Garlet, Gustavo P. ^[3] ; de Souza, Raphael F. ^[4] ; Taba, Jr., Mario ^[1] ; Sato, Sandra ^[4] ; de Souza, Sergio L. S. ^[1] ; Palioto, Daniela B. ^[1] ; Grisi, Marcio F. M. ^[1] ; Feres, Magda ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bucco Maxillo Facial Surg & Traumatol & Peri, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, BR-14040904 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Dept Dent Mat & Prostheses, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[5] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 26, n. 3, p. 359-367, MAY 2011.
• Citações Web of Science: 27

Resumo

The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP), or with the association of treatments (SRP + aPDT). Plaque samples were collected at baseline, 1, 3, and 4 weeks, and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-alpha), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16 S rRNA gene were evaluated through real-time PCR. The results shows that the levels of the majority of the species were reduced 1 week post-therapy for all treatments, however, an increase in counts of Prevotella intermedia (p = 0.00), Prevotella. nigrescens (p = 0.00) and Tannerella forsythia (p = 0.00) was observed for aPDT and SRP + aPDT. After 4 weeks, a regrowth of Porphyromonas gingivalis (p = 0.00) and Treponema denticola (p = 0.00), was observed for all treatments. Also, a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (p = 0.00). For the cytokine pattern, the results were similar for all treatments, and a reduction in the expression of cytokines and bacterial load was observed throughout the study. Our results suggest that SRP, aPDT in a single application, and SRP + aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis. (AU)

• Processo FAPESP: 05/60775-0 - Efeito da terapia fotodinamica antimicrobiana no tratamento da doenca periodontal e perimplantar. estudo de caes.
• Beneficiário: Rafael Ramos de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado