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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. Microbiological profile and cytokine pattern in the dog mandible

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Author(s):
de Oliveira, Rafael Ramos [1] ; Novaes, Jr., Arthur Belem [2, 1] ; Garlet, Gustavo P. [3] ; de Souza, Raphael F. [4] ; Taba, Jr., Mario [1] ; Sato, Sandra [4] ; de Souza, Sergio L. S. [1] ; Palioto, Daniela B. [1] ; Grisi, Marcio F. M. [1] ; Feres, Magda [5]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Dept Bucco Maxillo Facial Surg & Traumatol & Peri, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, BR-14040904 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Dept Dent Mat & Prostheses, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[5] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Lasers in Medical Science; v. 26, n. 3, p. 359-367, MAY 2011.
Web of Science Citations: 27
Abstract

The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP), or with the association of treatments (SRP + aPDT). Plaque samples were collected at baseline, 1, 3, and 4 weeks, and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-alpha), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16 S rRNA gene were evaluated through real-time PCR. The results shows that the levels of the majority of the species were reduced 1 week post-therapy for all treatments, however, an increase in counts of Prevotella intermedia (p = 0.00), Prevotella. nigrescens (p = 0.00) and Tannerella forsythia (p = 0.00) was observed for aPDT and SRP + aPDT. After 4 weeks, a regrowth of Porphyromonas gingivalis (p = 0.00) and Treponema denticola (p = 0.00), was observed for all treatments. Also, a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (p = 0.00). For the cytokine pattern, the results were similar for all treatments, and a reduction in the expression of cytokines and bacterial load was observed throughout the study. Our results suggest that SRP, aPDT in a single application, and SRP + aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis. (AU)