Prognostic Impact of MYCN, DDX1, TrkA, and TrkC Gene Transcripts Expression in Neuroblastoma

Autor(es)

Valadao de Souza, Daniela Raguer ; Sanabani, Sabri Saeed ^[1] ; Mamana Fernandes de Souza, Ana Carolina ; Filho Odone, Vicente ^[2] ; Epelman, Sidnei ^[3] ; Bendit, Israel ^[4]

Afiliação do(s) autor(es)

^[1] Fundacao Prosangue, Hemoctr Sao Paulo, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Crianca, Fac Med, BR-05403000 Sao Paulo - Brazil

^[3] Hosp Santa Marcelina Sao Paulo, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Hematol, Lab Biol Tumoral, Fac Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PEDIATRIC BLOOD & CANCER; v. 56, n. 5, p. 749-756, MAY 2011.
• Citações Web of Science: 9

Resumo

Background. The aims of this study were to define the mRNA expression profiles of MYCN, DDX1, TrkA, and TrkC in biopsy tumor samples from 64 Brazilian patients with neuroblastomas of different risk stages and to correlate altered expression with prognostic values. Procedure. Patients were retrospectively classified into low- (n = 11), intermediate- (n = 18), and high-risk (n = 35) groups using standard criteria. The mRNA levels of the above genes were measured by quantitative real-time polymerase chain reaction. Univariate analyses were performed and survival curves were plotted by the Kaplan-Meier method. Results. Of the 64 patients, 53% were female and 62.5% were older than 18 months. The 5-year overall survival (OS) for the entire cohort was 40.3%, with inferior median OS in patients identified in the intermediate- and high-risk groups. A significant difference in OS with respect to TrkA mRNA expression was found for the high-risk group vs. either the low- or intermediate-risk groups (P < 0.01, log rank test). Within the intermediate-risk group, neuroblastoma patients with positive TrkA mRNA expression had better clinical outcomes than patients with no TrkA transcript expression (P = 0.004). Another difference in OS was only found between the intermediate- and high-risk groups (P < 0.027, log rank test). No significant correlation of mRNA expression and survival outcome could be detected for the MYCN, DDX1. Conclusions. Positive expression of TrkA mRNA may be a clinically useful addition to the current risk classification system, allowing the identification of NB tumors with favorable prognosis. Pediatr Blood Cancer 2011; 56: 749-756. (c) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 03/09696-6 - Caracterização molecular dos neuroblastomas através da amplificação dos genes MYCN, DDX1 e NAG e da expressão em nivel de RNA de MYCN, TRK-A e DDX1: estudo retrospectivo de amostras provenientes de pacientes acompanhados no Protocolo Neuro IX-2000
• Beneficiário: Israel Bendit
• Modalidade de apoio: Auxílio à Pesquisa - Regular