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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster

Texto completo
Autor(es):
Pereira, G. B. [1] ; Valente, V. [1, 2] ; de Queiroz, M. S. [1] ; Vianna, M. C. B. [1] ; Paco-Larson, M. L. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FMRP, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, FMRP, Dept Cirurgia & Anat, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 44, n. 4, p. 283-290, APR 2011.
Citações Web of Science: 1
Resumo

Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3` of kermit (also called dGIPC). This mutant line presents a high lethality rate resulting from a gain of function. To obtain some insight into the biological role of the mutated locus, we have characterized the mutation and its relation to the high mortality of the KG00562 fly line. In this mutant, we did not detect one of the DmLpin transcripts, namely DmLpinK, but we did detect an unusual 2.3-kb mRNA (LpinK-w). Further investigation revealed that the LpinK-w transcript results from an aberrant splicing between the untranslated first exon of DmLpinK and the mini-white marker gene. Lack of DmLpinK or LpinK-w expression does not contribute to lethality, since heterozygous KG00562/Def7860 animals presented lethality rates comparable to those of the wild type. In contrast, the overexpression of kermit was associated with lethality of the KG00562 fly line. Significantly higher levels of kermit were detected in the Malpighian tubules of KG00562/+ flies that presented higher lethality rates than wild-type or KG00562/Def7860 animals, in which the lethality was rescued. In agreement with a recently reported study, our data support the hypothesis that misexpression of kermit/dGIPC could interfere with Drosophila development, with further investigations being needed in this direction. (AU)

Processo FAPESP: 07/52871-4 - Caracterização das alterações moleculares geradas pela mutação associada com a letalidade da linhagem kg00562 de Drosophila melanogaster
Beneficiário:Gustavo Borges Pereira
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 01/07696-3 - Caracterização de novos genes de Drosophilla melanogaster
Beneficiário:Valeria Valente
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 07/50173-8 - Estrutura e função de novos genes de Drosophila melanogaster cujos ortólogos em mamíferos são associados a disfunções celulares e teciduais
Beneficiário:Maria Luisa Paçó-Larson
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/52870-8 - Análise do padrão celular de expressão das proteínas dATX2 e dA2BP1/FOX-1 durante o desenvolvimento da retina de Drosophila melanogaster
Beneficiário:Mariana Santos de Queiroz
Linha de fomento: Bolsas no Brasil - Mestrado