| Texto completo | |
| Autor(es): Mostrar menos - |
Uombe, Nelsa Paula Inacio
;
Velikkakam, Teresiama
;
Silveira, Anna Clara Azevedo
;
Rodrigues, Cassiano Costa
;
Borges, Bruna Cristina
;
Teixeira, Thaise Lara
;
Pereira, Cecilia Luiza
;
Servato, Joao Paulo Silva
;
Melo, Normanda Souza
;
Mortara, Renato Arruda
;
da Silveira, Jose Franco
;
da Silva, Claudio Vieira
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROBIOLOGYOPEN; v. 14, n. 6, p. 6-pg., 2025-11-10. |
| Resumo | |
We characterized the secreted Trypanosoma cruzi P21 protein and hypothesized its role in parasite invasion and multiplication. To investigate the role of T. cruzi P21 protein in host-parasite interactions, specifically focusing on the low-virulence G strain. P21 knockout parasites were generated using CRISPR/Cas9. Cell invasion, multiplication, egress, and tissue parasitism were assessed in vitro and in vivo, comparing knockout and control parasites. P21 knockout significantly reduced parasite invasion and multiplication in Vero cells. In vivo, knockout parasites also showed reduced heart tissue parasitism in infected mice, despite no observable systemic parasitemia. Accordingly, P21 knockout trypomastigote egress was reduced in Vero cells. P21 plays a pleiotropic role in T. cruzi infection, differentially impacting parasite biology in the low-virulent G strain. In the G strain, P21 promotes invasion and persistence, potentially through mechanisms distinct from its role in the Y strain previously described. This highlights its potential as a therapeutic target for Chagas disease, warranting further investigation into strain-specific functions. (AU) | |
| Processo FAPESP: | 16/15000-4 - Trypanosoma cruzi: variabilidade genômica intra- e interespecífica e mecanismos de invasão/evasão celular |
| Beneficiário: | Renato Arruda Mortara |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/05049-4 - Construção de linhagens transgênicas modificadas por CRISPR/Cas9 para identificação de eventos de aneuploidia em Trypanosoma cruzi |
| Beneficiário: | Thaise Lara Teixeira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |