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Luchs, Adriana ; Adiwardana, Natanael Sutikno ; da Rocha, Leonardo Cecilio ; Viana, Ellen ; Guadagnucci, Simone ; Parise, Adriana ; Silva, Vanessa Cristina Martins ; de Azevedo, Lais Sampaio ; Guiducci, Raquel ; Franca, Yasmin ; Frank, Natacha Luana Pezzuol ; da Silva, Ana Lucia Nascimento ; Oliveira, Andre Luiz Vianna de ; Azevedo, Andre Henrique Souza ; Carreteiro, Barbara Segatelli ; Nogueira, Mauricio Lacerda

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 17, n. 4, p. 16-pg., 2025-03-29.

Resumo

Pneumonia and diarrhea are the leading causes of death in children under 5 globally, worsened by viral infections. This study investigates viral agents in children <= 3 years with respiratory illness and diarrhea in Metropolitan Region of S & atilde;o Paulo, Brazil, during spring 2021. Twenty paired samples (oropharyngeal swab and feces) were tested using in-house qPCR for HBoV and HAdV, RT-qPCR for RVA, EV, PeV-A, and NoV, and a commercial RT-qPCR kit for SARS-CoV-2, Flu A/B, and RSV. HAstV was detected with conventional nested (RT)-PCR. Positive samples were sequenced for molecular characterization and phylogenetic analysis. Seven viruses were identified: HBoV, NoV, HAdV, PeV-A, EV, RSV, and Flu A. HBoV and NoV were detected in 75% of cases, with co-infection in 65% of patients, indicating their involvement in the gastro-respiratory illness. Genotyping of HBoV (HBoV-1), NoV (GII.4_Sydney[P16], GII.2[P16], and GII.4_Sydney[P31]), EV (Coxsackievirus A6), HAdV (species C, type 6), and PeV-A (genotype 1) showed local virus diversity. Phylogenetic analysis indicated no ongoing community outbreak, with distinct clusters observed. The findings highlight the overlap of respiratory and enteric diseases, revealing local viral diversity and high exposure to enteric viruses. This underscores the challenges in differential diagnosis and the need for syndromic surveillance. (AU)

• Processo FAPESP: 15/12944-9 - Investigando a evolução de cepas animais de rotavírus infectando humanos
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/14786-0 - Vigilância e epidemiologia molecular dos Bocavirus humanos associados à gastroenterite aguda
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/02529-8 - Epidemiologia molecular dos bocavírus humanos em amostras fecais históricas no brasil (1998-2005)
• Beneficiário: Ellen Viana de Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 21/09064-8 - Vigilância e epidemiologia molecular dos Bocavírus humanos associados à gastroenterite aguda
• Beneficiário: Yasmin França Viana Pires de Souza
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico