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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Highly Toxic Microcystis aeruginosa Strain, Isolated from So Paulo-Brazil, Produce Hepatotoxins and Paralytic Shellfish Poison Neurotoxins

Texto completo
Autor(es):
Sant'Anna, Celia L. [1] ; de Carvalho, Luciana R. [1] ; Fiore, Marli F. [2] ; Silva-Stenico, Maria Estela [2] ; Lorenzi, Adriana S. [2] ; Rios, Fernanda R. [1] ; Konno, Katsuhiro [3] ; Garcia, Carlos [4] ; Lagos, Nestor [4]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Inst Bot, Secao Ficol, BR-04301012 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba - Brazil
[3] Ctr Toxinol Aplicada, Inst Butantan, Sao Paulo - Brazil
[4] Univ Chile, Fac Med, Dept Fisiol & Biofis, Lab Bioquim Membrana, Santiago 7 - Chile
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: NEUROTOXICITY RESEARCH; v. 19, n. 3, p. 389-402, APR 2011.
Citações Web of Science: 18
Resumo

While evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic shellfish poison neurotoxins, the hydrophilic extract of Microcystis aeruginosa strain SPC777-isolated from Billings's reservoir, So Paulo, Brazil-was found to exhibit lethal neurotoxic effect in mouse bioassay. The in vivo test showed symptoms that unambiguously were those produced by PSP. In order to identify the presence of neurotoxins, cells were lyophilized, and the extracts were analyzed by HPLC-FLD and HPLC-MS. HPLC-FLD analysis revealed four main Gonyautoxins: GTX4(47.6%), GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC-MS analysis, on other hand, confirmed both epimers, with positive Zwitterions M(+) 395.9 m/z for GTX3/GTX2 and M(+) 411 m/z for GTX4/GTX1 epimers. The hepatotoxins (Microcystins) were also evaluated by ELISA and HPLC-MS analyses. Positive immunoreaction was observed by ELISA assay. Alongside, the HPLC-MS analyses revealed the presence of {[}l-ser(7)] MCYST-RR. The N-methyltransferase (NMT) domain of the microcystin synthetase gene mcyA was chosen as the target sequence to detect the presence of the mcy gene cluster. PCR amplification of the NMT domain, using the genomic DNA of the SPC777 strain and the MSF/MSR primer set, resulted in the expected 1,369 bp product. The phylogenetic analyses grouped the NMT sequence with the NMT sequences of other known Microcystis with high bootstrap support. The taxonomical position of M. aeruginosa SPC777 was confirmed by a detailed morphological description and a phylogenetic analysis of 16S rRNA gene sequence. Therefore, co-production of PSP neurotoxins and microcystins by an isolated M. aeruginosa strain is hereby reported for the first time. (AU)

Processo FAPESP: 05/56303-5 - Caracterizacao molecular de cianobacterias brasileiras e selecao de linhagens produtoras de peptideos nao ribossomicos.
Beneficiário:Marli de Fátima Fiore
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/16042-5 - Nanotecnologia: observação das estruturas formadas pela interação entre peptídeos não-ribossômicos, mutantes de metalotioneínas e lotioneinas e metais
Beneficiário:Maria Estela Stenico
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado