| Texto completo | |
| Autor(es): |
Alessio, Priscila
;
Martin, Cibely S.
;
Maximino, Mateus D.
;
Pereira, Matheus S.
;
Apetrei, Constantin
;
Rodriguez-Mendez, Maria L.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Food Engineering; v. 410, p. 8-pg., 2026-05-01. |
| Resumo | |
The immobilization of enzymes such as Tyrosinase (Tyr) in biomimetic nanostructured films is a promising approach to producing highly sensitive and selective biosensors for detecting phenolic compounds. In this study, an innovative approach to preserve the enzymatic functionality in a biomimetic environment is presented. Tyr was encapsulated within liposome structures formed by a lipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) using a straightforward technique, resulting in a grape-shaped morphology. The lutetium bisphthalocyanine (LuPc2) was also added to the liposome structure as electrocatalytic material and structured as thin film using the layer-by-layer (LbL) technique for biosensor application. The biosensor shows a Hill coefficient of 0.986 and a Michaelis-Menten constant of 21.96 mu M, and a limit of detection of 44 nM for catechol detection. Measurements of polyphenolic contents performed in grape juice samples reveal that the encapsulation of Tyr inside liposomes preserves the functionality and improves the electrochemical performance for amperometric and voltametric detections. (AU) | |
| Processo FAPESP: | 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico |
| Beneficiário: | Osvaldo Novais de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/14235-6 - Modelos miméticos de membrana aplicados em estudos de interação, detecção e remoção de poluentes emergentes |
| Beneficiário: | Priscila Alessio Constantino |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 23/14967-2 - Materiais nanoestruturados aplicados como plataforma sensoriais via (espectro)eletroquímica para a detecção de amostras de interesse ambiental. |
| Beneficiário: | Cibely da Silva Martin Sonvesso |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |